Our parts that can count as contributions include:
• K4494000 (mApple): A new reporter, mApple, which is significantly brighter than many other variants, and therefore could be used by future iGEM teams in need.
• K4494001 (csoR): A new repressor that was documented with extensive experimental data.
• K4494002 (PIII) and K4494196 (PIII+RBS): Respectively a new promoter and its corresponding promoter-RBS composite part. They were also newly created and documented parts for use by future iGEM teams.
• K4494003-K4494098: A new collection of PIII+RBS variants that is well-documented and could be used and/or explored freely by future interested teams or all interested researchers in general.
• K1980004 (pCusC): This is an already-existing iGEM part which we characterized and constructed variants (K4494099-K4494102) for. Specifically, K4494102 is a newly found exceptionally strong promoter.
• K4494197 (Doc): This is a toxin that we found and synthesized during the early stages of our project. Though we eventually did not use it, we reserved our synthesized sample for future iGEM teams to use. It is labeled part C0001D in the Northwest University Part Library.
On our Model page, we presented two models. The first one is a theoretical calculation of the relationship between promoter activity and copper concentration in our project, in which we took toxicity-induced cell death into account. Due to a lack of data, we did not further investigate our experimental data with this model. However, it still led to many insights regarding our system, and we constructed an interactive demonstration of this model on our wiki page so that other teams can also use it. For more information please refer to our Model page.
Our student leader Shiran Yuan made correction efforts to Chinese translations to the iGEM SynBio Translation Glossary and thus he was invited by iGEM to be an official volunteer translator.
In order to support biosafety, we designed and 3D printed a biosafe box hardware prototype. It consists of two parts: one to place sample tubes in and another to cultivate strains in. The 16 larger wells are used to support sample tubes so that they can be carried in the box, back to places such as the laboratory. The 49 smaller ones are used to directly place strains in. In case of leakage of the strains, the box lid closely fits with the strain cultivation wells so that the strains are safely kept, and can be directly disposed of after the experiments are completed in the wild. For more information on this (and the document on how to make the box) please visit our Proposed Implementation page.
JLU_China led the creation of 2022: An Instruction Handbook for New Teams, a handbook intended for helping new iGEM teams through various stages of their project. We, DKU_China, contributed to the handbook by writing the article A Quick Guide to 3D Modeling for iGEM, an article for teams who have completely no prior knowledge of 3D Modeling. We hoped that it could empower those iGEM teams to realize their thoughts on hardware design to solve any potential problems they might have.
An Instruction Handbook for New Teams