| Parts name | Parts number | Types | length（bp） | website links |
|---|---|---|---|---|
| hrpR | BBa_K4226001 | Coding | 945 | http://parts.igem.org/Part:BBa_K4226001 |
| hrpS | BBa_K4226002 | Coding | 909 | http://parts.igem.org/Part:BBa_K4226002 |
| hrpL Promoter | BBa_K4226003 | Regulatory | 208 | http://parts.igem.org/Part:BBa_K4226003 |
Those parts above are improvement for the parts：HrpR Gene (BBa_K1014001), HrpS Gene (BBa_K1014000) and Promoter hrpL (BBa_K1014002) uploaded by iGEM13_HIT-Harbin. The sequences are different from what they uploaded, and it's been quantitatively verified.
In our project，we designed the Amp30E amplification device consists of hrpR (BBa_K4226001), hrpS (BBa_K4226002) , and hrpL promoter (BBa_K4226003) . HrpR protein binds to hrpS protein forming a complex, and the promoter hrpL (BBa_K4226003) is transcriptionally upregulated by hrpR (BBa_K4226001) and hrpS (BBa_K4226002) [1].
We characterized these new parts and added new documentation to it. We hope this will make a contribution to the iGEM community.