TEAM
Member
Attributions
Partnership
Collaborations
PROJECT
Description
Design
Implementation
Contribution
Safety
PARTS
Part Collection
New Parts
Measurement
Improvement
EXPERIMENTS
Results
Model
Hardware
Proof of Concept
Engineering
Protocol
Notebook
HUMAN PRACTICES
Integrated Human Practices
Education
Inclusivity
Communication
Entrepreneurship
MEDALS
Non-specific Part
Quorum Sensing Part
Biological Scaffold
Biosafety
Calcium Carbonate Production
Protocol
Non-specific Part
E.coli transformation
Preparation of chemically competent state by TSS method
6*His tag protein purification
Western blotting
Overlaping PCR
Seamless clone
Bacillus subtilis Transformation (Use protocol from iGEM LMU-Munich 2014)
Quorum Sensing Part
Zone of subtilisin inhibition Experiment
Gradient concentration of subtilisin in individual plates
Plates with subtilisin to trigger signaling part
Culture with subtilisin of WB600 transformed by signaling plasmid
Culture with low-oxygen condition of WB600 transformed by subtilisin production plasmid
Biological Scaffold
IPTG induced the expression of PET series plasmid proteins
Immunoprecipitation of HA tag fusion protein（beyotime HA Tag Protein ImmunoPrecipitation Kit）
6*His tag protein purification
SYTO Green Live cell nucleic acid dye
Biosafety
SDS-page detection qualitative analysis
Bacillus subtilis genome DNA
Bacillus subtilis sucrose induced expression
Calcium Carbonate Production
CA plasmid transformation and expression
CO
2
hydration catalytic activity of CA
CaCO
3
estimation
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