In this year, we created a part collection used for biomineralization experiments with Bacillus subtilis chassis. Among them, BBa_K2232000, BBa_K4202009 are used to accelerate calcium carbonate production and precipitation; BBa_K4202006, BBa_K4202015 are used to construct self-assembled extracellular bio-scaffolds; BBa_K4202002 and BBa_ K4202003 are a pair of fluorescent probes for characterizing the formation of bio-scaffolds. Each of these parts has been well characterized and documented at Part Registry.
| Name | Length(bp) | Type | Function and description | Designer |
|---|---|---|---|---|
| BBa_K4202004 | 837 | Coding | Coding sequence of carbonic anhydrase condon-optimized for Bacillus subtilis | Junyi Liu |
| BBa_K4202002 | 786 | Coding | This part is a fusion protein of mRFP and a short peptide named SpyTag | Zihang Ye |
| BBa_K4202003 | 1110 | Coding | This part is a fusion protein of mRFP and a engineered protein named SpyCatcher | Zihang Ye |
| BBa_K4202006 | 954 | Coding | This part is a fusion protein of Hag and SpyTag , and it`s used for the bio-scafford | Zihang Ye |
| BBa_K4202009 | 681 | Coding | Amorphous Calcium Carbonate Binding Protein(ACCBP)(with signal peptide) | Hongyin Lin |
| BBa_K4202015 | 771 | Coding | This part is a fusion protein of EutM and SpyCatcher , and it`s used for the bio-scafford | Yuyan Su |
