Notebook for experiment
2022.8.10
Team Ice-breaking
Project discussion
Determination of team’s name and logo
Project discussion
Determination of team’s name and logo
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2022.8.11
Safety training in laboratory
LB Culture Medium cultivation
YPD Culture Medium cultivation
PCR: lac-12 & wbgl, gmd & wcaG
Culture solution configuration
LB Culture Medium cultivation
YPD Culture Medium cultivation
PCR: lac-12 & wbgl, gmd & wcaG
Culture solution configuration
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2022.8.12
Gel electrophoresis
Gel extraction
Plasmid extraction
Transformation
Gel extraction
Plasmid extraction
Transformation
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2022.8.13
Verification of bacteria colony by PCR
Gel electrophoresis
Gel electrophoresis
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2022.8.14
Cultivation of Yeast competent cells
Enzyme digestion
Enzyme digestion
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2022.8.15
Reintroduce the aim of our experiment
YPD30L2 cultivation
YPD30L2 cultivation
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2022.8.16-17
Verification of S. cerevisiae colony by PCR
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2022.8.18-23
Curve of the growth of Saccharomyces cerevisiae
Test 2’-FL content
Test 2’-FL content
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