Parts created by our team.
We contributed two new enzymes to the iGEM registry: fluoroacetate dehalogenase (FAcD) and haloacid dehalogenase (HAD). Both enzymes have been noted to have deflorinating functions. Additionally, utilizing 3 iGEM registry parts (BBa_J23119, BBa_B0034, BBa_B0010) we created the composite parts incorporating our basic parts as the enzyme, with the purpose of degrading PFAS in a water system. In addition to having the common function of cleaving C-F bonds, all parts have been modified to be compatible for a U loop system (loop assembly). Our part collection can help and inspire future teams to further design parts using loop assembly and different degradation enzymes.
| Name | Type | Description | Designers | Length |
|---|---|---|---|---|
| BBa_K4191004 | Coding HAD | Haloacid dehalogenase enzyme CDS, derived from Rhodopseudomonas palustri, codon-optimized for E. coli, includes C-D overhangs compatible with PCA odd-1 backbone for u loop system | Madison Yang, Ananya Bharathwaj | 796 bp |
| BBa_K4191001 | Coding FAcD | Fluoroacetate dehalogenase enzyme CDS, derived from Xanthobacter autotrophicus, codon-optimized for E. coli, includes C-D overhangs compatible with PCA odd-1 backbone for u loop system | William Huynh, Swasti Singhai | 943 bp |
| BBa_K4191002 | Composite Part | engineered PJ23119+RB0034+FacD+TB0010, includes iGEM designed promoter, RBS, and terminator, along with the FAcD coding sequence (BBa_K4191001) | Kavya Gupta, Ananya Bharathwaj | 1191 bp |
| BBa_K4191003 | Composite Part | PJ23119+RB0034+HAD+TB0010, includes iGEM designed promoter, RBS, and terminator, along with the HAD coding sequence (BBa_K4191002) | Anika Yadav, Fiona Zhang | 1044 bp |