The purpose of our project is to help facilitate the screening and prognosis of breast cancer for ordinary people at home. We developed a detection kit for two circRNA biomarkers, hsa_circ_0001785 and hsa_circ_0001982, of breast cancer. They are the target circRNAs for detection in this project. The kit is a cell-free system containing all molecular machineries needed for a CRISPR-Cas12a-based detection process. In this project, we have successfully constructed 8 composite parts and 10 basic parts for igRNAs and dsDNAs .

No	Name	Type	Description	Length
1	BBa_K4409000	Basic	gRNA / crRNA fixed-loop repeat for Cas12a	23bp
2	BBa_K4409001	Basic	guide of igRNA (hsa_circ_0001785)	20bp
3	BBa_K4409002	Basic	guide of igRNA (hsa_circ_0001982)	21bp
4	BBa_K4409003	Basic	trigger-RNA sensor of igRNA (hsa_circ_0001785)	43bp
5	BBa_K4409004	Basic	trigger-RNA sensor of igRNA (hsa_circ_0001982)_Coupled with GAPDH as guide sequence	43bp
6	BBa_K4409005	Basic	trigger-RNA sensor of igRNA (hsa_circ_0001982)_coupled with hsa_circ_0001785 as the guide sequence	43bp
7	BBa_K4409006	Basic	dsDNA (GAPDH)	27bp
8	BBa_K4409007	Basic	dsDNA(hsa_circ_0001785)	24bp
9	BBa_K4409008	Basic	dsDNA(hsa_circ_0001982)	25bp
10	BBa_K4409009	Composite	igRNA (AAG+GAPDH as guide+ hsa_circ_0001982 as trigger)	86bp
11	BBa_K4409010	Composite	igRNA (CT+GAPDH as guide+ hsa_circ_0001982 as trigger)	86bp
12	BBa_K4409011	Composite	igRNA (CT+ hsa_circ_0001785 as guide+ hsa_circ_0001982 as trigger)	84bp
13	BBa_K4409012	Composite	igRNA (CT+ hsa_circ_0001982 as guide+ hsa_circ_0001785 as trigger)	87bp
14	BBa_K4409013	Composite	gRNA (CT+GAPDH as guide)	43bp
15	BBa_K4409014	Composite	gRNA (CT+ BBa_K3859000)	50bp
16	BBa_K4409015	Composite	gRNA (AAG+ BBa_K3859000)	51bp
17	BBa_K4409016	Composite	gRNA (AAG+ GAPDH as guide)	44bp
18	BBa_K4409017	Basic	guide of igRNA (GAPDH)	23bp