Parts
Parts
We use and construct parts that are considered an
important part of our project, and most of them have been tested to ensure their
reliability.
To efficiently obtain these parts, we asked
Tsingke Biotechnology to synthesize
some of them. Besides, we also used colony PCR to get several parts from the
original bacteria strain directly.
You can click Design to learn more about our
project to see their specific
function.
Basic Parts
Among the basic parts designed this year by ourselves,
BBa_K4144004 and BBa_K4144011 are our favorite. BBa_K4144004 is a
codon-optimized eukaryotic protein, which transports SAMe from engineered
bacterial cytosol to extracellular environment. BBa_K4144011, an optimized SAMe
synthetase which will not be regulated by feedback inhibition, is utilized to
improve Sam2 expression efficiency in gram-positive bacteria.
Basic Parts
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_K1074012 | Pgrac | Promoter | 170bp |
| BBa_K143014 | Pxyl | Promoter | 82bp |
| BBa_K2985006 | P43 | Promoter | 445bp |
| BBa_K143021 | SpoVG RBS | RBS | 12bp |
| BBa_K4144001 | RBS_grac | RBS | 10bp |
| BBa_K780000 | Termiantor | Terminator | 41bp |
| BBa_K4144002 | Terminator_pBE | Terminator | 80bp |
| BBa_K4144011 | opSam2 | CDS | 1158bp |
| BBa_K4144004 | opPet8p | CDS | 852bp |
| BBa_K4144005 | opMistic | CDS | 330bp |
| BBa_K3286203 | sfGFP | CDS | 717bp |
| BBa_K3498008 | XylR | CDS | 1053bp |
| BBa_K4144009 | linker | CDS | 30bp |
| BBa_C0076 | CinI | Coding | 727bp |
| BBa_K1893028 | CinR | Coding | 746bp |
| BBa_R0077 | CinR promoter | Promoter | 231bp |
| BBa_K2447012 | TlpA36 | Coding | 1116bp |
| BBa_K2447011 | pTlpA36 | Promotor | 52bp |
| BBa_C0074 | Penl repressor | Coding | 448bp |
| BBa_R0074 | pPenl | Promoter | 77bp |
| BBa_K1054002 | PBSX holin | Coding | 264bp |
| BBa_E0040 | EGFP | Coding | 757bp |
| BBa_K143013 | P43 | Promoter | 56bp |
| BBa_R0010 | Placi | Promoter | 200bp |
| BBa_B0034 | RBS | RBS | 12bp |
| BBa_E1010 | mRFP1 | CDS | 706bp |
| BBa_B0015 | double terminators | Terminator | 129bp |
| BBa_K322921 | sacB | CDS | 1437bp |
| BBa_J23116 | Pc | Promoter | 35bp |
| BBa_C0012 | lacl | CDS | 1153bp |
| BBa_K4144040 | KanR+RBS | CDS | 860bp |
| BBa_K4144041 | lactose-tolerant lacl repressor | CDS | 1163bp |
| BBa_K4144012 | improved RBS | RBS | 12bp |
Composite Parts
We have designed a series of composite parts this
year and most of them have been tested. BBa_K4144081 and BBa_K4144082 are for
the verification using EGFP of chemical switch and temperature switch
respectively. BBa_K4144006 is for reducing the leakage of Pxyl and BBa_K4144008
is for synthesizing SAMe. BBa_K4144007 and BBa_K4144010 are for transporting
SAMe. BBa_K3482025 is for oscillation of SAMe.BBa_K4144044 and BBa_K4144043 are
for double selection and improved expression of LacI respectively. BBa_K143057
is used to express our SAMe transporter opPet8p.
Composite Parts
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_K4144081 | P43+CinR+CinR promoter+terminator+EGFP | Composite | 1775bp |
| BBa_K4144082 | P43+TlpA36+terminator+pTlpA36+RBS(BBa_K104001)+Penl+pPenl+RBS+EGFP | Composite | 2503bp |
| BBa_K4144006 | P43+XylR+terminator_pBE | Generator | 1592bp |
| BBa_K4144007 | Pxyl-SpoVG RBS-6xHis tag-opMistic -3xFLAG-opPet8-sfGFP-terminator | Composite | 404bp |
| BBa_K4144008 | Pgrac+RBS_grac+6xHis+opSam2+terminator | Composite | 404bp |
| BBa_K4144010 | opMistic+opPet8p+linker+sfGFP | Coding | 2211bp |
| BBa_K3482025 | PLtet01 promoter-mVenus-CFP-AmpR promoter-AmpR-pSC101 ori-Rep101-lambda repressor-PLtet01 promoter-LacI-TetR-pL Lac01 | Plasmid | 8702bp |
| BBa_K4144043 | Pc+ RBS+lacI+ssA tag(LVA) | Composite | 1328bp |
| BBa_K143057 | Pxyl-RBS-spoVG | Generator | 102bp |
| BBa_K4144044 | lac promoter+ mRFP +sacB+ KanR + terminator | Composite | 3344bp |