ATTRIBUTIONS

Introduction

Each team member had a formal role within the team and responsibilities within the project. However, the end result of Project SPYKE could not have been achieved without the support of many external parties such as supervisors, advisors, experts, and stakeholders who guided us on different aspects of the project. Furthermore, none of our work could have been possible without the support of our sponsors. Here, we would like to summarize each and everyone’s contribution to the project.

At TU Delft, iGEM is accounted for as a course. Therefore, students can apply for it and consequently join the competition, guided by several instructors and advisors. After the selection process, we started with a brainstorming phase to determine the main ideas for the project to grow. This phase extended until April when we chose to create SPYKE. The following months were then dedicated to giving structure to the project. We focused on both the scientific and societal side of the project, as well as fundraising, outreach, and education. For this, we assembled teams to better manage specific sections of the project. The table (Table 1) underneath reports a clear overview of every team member’s work:

TEAM CONTRIBUTIONS

Project SPYKE could not have been possible without external support from our supervisors and other experts. In the next section, we would like to express our gratitude for their help and their support.

SUPERVISORS

Principal Investigator

Dr. Christophe Danelon - Associate professor at Dept. of Bionanoscience, TU Delft, The Netherlands - for advising us on every aspect of the project and guiding us throughout the process, and for teaching us about cell-free systems

Supervisors

Britte Bouchaut - Postdoctoral researcher at Dept. of Biotechnology, TU Delft, The Netherlands

Ana Maria Restrepo Sierra - Ph.D. candidate at Dept. of Bionanoscience, TU Delft, The Netherlands

Esengül Yildirim - Research technician at Dept. of Bionanoscience, TU Delft, The Netherlands

Justas Ritmejeris - Ph.D. candidate at Dept. of Bionanoscience,TU Delft, The Netherlands

for advising us on every aspect of the project and guiding us throughout the process, and for giving us the required lab training

Ellen Winkler - Academic counselor at TU Delft - for providing coaching for efficient project management and teamwork

SCIENCE CONTRIBUTIONS

DESIGN AND RESEARCH

Alexander Gräwe - Postdoctoral researcher at TU Eindhoven, The Netherlands - for talking to us about his experience on making a biosensor for GHB in the 2015 iGEM Bielefeld team

Prof. Marc Ostermeier - Professor of chemical and biomolecular engineering, Johns Hopkins University, USA - for discussing with us allosteric fusion protein engineering

Dr. Frans Widdershoven - Professor of bioelectronics at TU Delft, The Netherlands - for teaching us about biosensors on a chip and the electronics behind such devices

Dr. Fioreno Omenetto - Professor at Dept. of Biomedical Engineering, Tufts University, USA - for advising us on a biosensor with silk, which was our initial idea for this project

Laura Koekkoek - Research technician at Dept. of Biotechnology, TU Delft, The Netherlands - for providing us with technical and logistical assistance in the laboratory

Dr. Peter-Leon Hagedoorn - Assistant professor at Dept. of Biotechnology, TU Delft, The Netherlands - for providing us with advice on techniques to measure DNA-protein binding affinity and ITC (Isothermal Titration Calorimetry )

Dr. Duncan McMillan - Assistant professor at Dept. of Biotechnology, TU Delft, The Netherlands & Dr. Humberto Sanchez Gonzalez - Research technician at Dept. of Bionanoscience, TU Delft, The Netherlands - for providing us with advice on DNA binding to a surface

Dr. Allard Katan - Research technician at Dept. of Bionanoscience, TU Delft, The Netherlands & Adja Zoumaro-Djayoon - Dept. of Bionanoscience, TU Delft, The Netherlands - for helping us using the AFM and interpreting the data

Angeliki Goutou - Research technician at Dept. of Bionanoscience, TU Delft, The Netherlands - for sharing with us her expertise on protein purification

Ilja Westerlaken - Research technician at Dept. of Bionanoscience, TU Delft, The Netherlands - for providing us with technical assistance on size exclusion chromatography

HUMAN PRACTICES

Dr. Lotte Asveld - Assistant Professor at Dept. of Biotechnology, TU Delft, The Netherlands - for teaching us about Value-Sensitive Design, Safe-By-Design, and Responsible Innovation

Wessel Teunisse & Cecile van der Vlught - employees of the RIVM (dutch institute of public health) - for helping us with the safety of our project and our Safe-by-Design approach

And a special thanks to everyone that agreed to be interviewed by us:

Steven Biemans - employee at Trimbos Institute, The Netherlands

Amber Schaafsma - first aid doctor in the MLC hospital, The Netherlands

Ronald van Litsenburg - owner of Event Medical Services

Querine Hoejenbos - employee of the "KNH"

Those who participated in our focus group

Everyone who filled in our surveys

Jetti Steffers - creative director of Perron

Lars Van Driel- part of the EHBDD (First aid expertise center for drinking and drugs accidents)

Cornelis - police officer

Erik Izaks - advisors of the public prosecutor in the Netherlands

Mette Rurup - forensic advisor for the police

Leanneart Borra - forensic toxicologist from the NFI

Nienke le Fever - forensic prosecutor at the Dutch public prosecution service (OM)

Aaldrik Krol - project leader specialized in drug crime at the Dutch National Police

Corine van Bethlehem - pharmacist specialized in toxicology at the Erasmus Medical Center hospital in Rotterdam

Femke Gresnigt - emergency physician specialized in toxicology at the OLVG hospital in Amsterdam

Marleen Voorhuijzen & Gijs Kleter - Wageningen Food Safety Research

Reinhilde Schoonjans - EFSA

Dita Kalfsbeek - NVWA

Bianca van de Ven - toxicologist and risk advisor at the RIVM

Ingrid Möllers & Cynthia van Rijn - Bureau GGO

Hidde Rang - VWS

The two victims that agreed to be interviewed by us

FINANCE AND ENTREPRENEURSHIP

Bram Henneman - V.O. patenting and trademarks & Irene Otten-Reynhout - Intellectual Property Manager at TU Delft Innovation & Impact Centre & Peter van Dongen - RVO - for advising us on how to patent our product

Annelies H. De Bosch & Kemper-de Hilster - V.O. patenting and trademarks - for helping us with NDAs

Blijke Kroezen - V.O. patenting and trademarks - for being our patent attorney and helping us filing for the patent

HARDWARE

Nemo Andrea - for helping us with the 3D design of our prototype

Dr. Margeet Docter - Associate professor at Dept. of Bionanoscience, TU Delft, The Netherlands - for advices on the overall electronics in this project

Dong Hoon Shin - TU Delft, The Netherlands - for giving us more information about downsizing our hardware

Jeroen Bastemeijer - TU Delft, The Netherlands - for helping us design the electrical system

Dr. Roland Kieffer - TU Delft, The Netherlands - for allowing us access to the BN makerspace and 3D printer

Dr. Gerard Meijer - Professor at Dept. of Microelectronics, TU Delft, The Netherlands - for advising us on measuring the capacitance change with UTI

Ir. Zu-Yao Chang - Dept. of Microelectronics, TU Delft, The Netherlands - for improving our UTI set-up, guiding us during the vector impedance measurements and overall advice on the electronics

Dr. Guijie Wang - Dept. of Microelectronics, TU Delft, The Netherlands - for advising us on measuring the capacitance change

Zulhaj Rizki - Ph.D. candidate at Dept. of Biotechnology, TU Delft, The Netherlands - for advice on the implementation of a membrane in our system

Ali Heidari - Dept. of Mechanical Engineering, Islamic Azad University - for advising us on working with the UTI and leaky capacitors

Jack Maat - TU Delft, The Netherlands - for general advice on electronics

Kees Esser - TU Delft, The Netherlands - for giving us support concerning the electronic parts of our project

Diederik la Haye - TU Delft, The Netherlands - for allowing us to use the Applied Physics maker’s space

Jeroen Lybaert - Metrohm - for advising us on which chip to buy

Paola de Magistris - Postdoctoral researcher at Dept. of Bionanoscience, TU Delft, The Netherlands - for advising us on QCM-D

Outreach

Doris Boschma - GameLab, The Netherlands - for helping us design our board game NUCLEO

Lotte Havik & Carlijn Voorwinde - for doing their high school project with us

Dimmy van Ruiten - TU Delft, The Netherlands - for giving us the media training and helping us with our press release

Sanne van den Broek - TU Delft, The Netherlands - for giving us the media training

Charlotte de Kort - TU Delft, The Netherlands - for helping us with our overall communication and press release

Jan Adriaanse - Leiden University, The Netherlands - speaking at our Entrepreneurship event

Cailin Greiner - YesDelft, The Netherlands - speaking at our Entrepreneurship event

Andreas Keymer - V.O. patenting and trademarks - speaking at our Entrepreneurship event

Nathan Douenburg - for helping us with the promotional video

Safety

Wim Best - Coordinating/specialist senior-inspector at Controlled Substances Unit, Health and Youth Care Inspectorate, The Netherlands - for clarification regarding working with SSA

Michiel Faber - TU Delft, The Netherlands - for helping regarding working with regulated substances

Tonny Schuit - TU Delft, The Netherlands - for helping regarding working with regulated substances

Others:

Department of Bionanoscience, TU Delft, The Netherlands for giving us workspaces, access to their lab facilities, administrative and financial support

Department of Biotechnology, TU Delft, The Netherlands for providing us with an ML-1 lab, workspaces, administrative and financial support

2021 TU Delft iGEM team for sharing their experience with us and providing advice regarding the competition

2015 Bielefeld iGEM team as our project is built upon their original idea

Jannick Weitzel for helping us with our website and our wiki

Stephen Eustace for helping us obtain chemicals

Pieter Oomen - Trimbos-instituut - for allowing us to analyze our system with GHB

Ruben Vrolijk - Trimbos-instituut - for guiding us through the experiments at Trimbos to analyze our system with GHB

Haroun Bensaadi for being part of the team in the initial development of the project