Notebook

A quick summary of 2022 iGEM Toronto journey. Details can be found in the Experiment page

Date Description
02.04.2022 - 07.08.2022 Project planning and designing
Compared different detection assays methods and selected LAMP
Designed multiple sets of LAMP primers
Ordered LAMP primers and reagents
08.08.2022 - 14.08.2022 Preparing reagents
Resuspended primers and DNA
Aliquoted reagents
LAMP Experiments
Preliminary primer set testing with NEB Warmstart colorimetric master mix
15.08.2022 - 21.08.2022 LAMP Experiments
Testing LAMP Primers with Lucigen Master Mix
22.08.2022 - 29.08.2022 LAMP Experiments
Testing LAMP Primers with Gel Electrophoresis verification
Troubleshooting Gel Electrophoresis contamination
29.08.2022 - 04.09.2022 LAMP Experiments
Testing Primer ratios
05.09.2022 - 11.09.2022 LAMP Experiments
LAMP test on qPCR machine for melting curve analysis
Freeze drying LAMP assay
12.09.2022 - 02.10.2022 Break from lab experiments
03.10.2022 - 10.10.2022 LAMP Experiments
LAMP test on qPCR machine for melting curve analysis