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Notebook

Week 1-10

Week 4

Project

1. Built up a team on January 24th.
2. Grouped online, with each group choosing their own direction for a project and there's an advisor in each group to help the new members.

Week 5

Project

1. Each group was very motivated during the iGEM journey and successively carried out their own project direction.

Week 6

Project

1. The five groups began to make presentation for their projects.
2. The advisors of each group supported and guided their presentation of the project.

Week 7

Project

1. Celebrated Chinese New Year.

Week 8

Project

1. Interpreted previous years' projects.
2. We brainstormed HP's activities.

Week 9

Project

1. The presentation of each group was completed, and the advisors gave suggestions for us on March 4th.

Week 10

Project

1. Added Synthetic Biology to our coursework and began to study.
2. Team members revised the presentation and began to prepare for the offline presentation.

Week 11-20

Week 11

Project

1. Determined the list of team members, and made an outline of HP part on March 16th .
2. The tasks of posting tweets were determined at the meeting on March 18th.

Week 12

Project

1. Determined the direction of the questionnaire on March 21st.
2. Posted "Synthetic Biology" on the public account on March 25th.
3. A meeting was held, the project interpretation report was conducted at the meeting on March 26th.

Week 13

Project

1. "Project Interpretation Series ①" was posted on the public account on March 30th.
2. Summarized the problems of tweets on April 3rd.
3. Discussed making a vocabulary list about synthetic biology.

Week 14

Project

1. "No distance to synthetic biology" was posted on the public account on April 4th.
2. "Project Interpretation Series ②" was posted on the public account on April 6th.
3. "The Beautiful World of Synthetic Biology" was posted on the public account on April 8th.
4. We watched the live broadcast of the synthetic biology competition which named "Symposium on Intersection of Structure, Cell and Synthetic Biology".

Week 15

Project

1. "Project Interpretation Series ③" was posted on the public account on April 13th
2. "what can we learn from synthetic biology" was posted on the public account on April 15th.
3. The seventh meeting was held on April 17th. At the meeting, we planned the activities of HP, shared the notebook and reviewed the work content; student leader emphasized the importance of team integration; members reported on project interpretation.

Week 16

Project

1. "Project Interpretation Series ④" was posted on the public account on April 20th.
2. "Synthetic Biology Is Changing the World" was posted on the public account on April 22nd.
3. Communicated with SZU and SUSTech April 22nd.

HP

1. Our team came to Watsons to interview salesmen on April 18th.

Week 17

Project

1. The project interpretation tweet was posted on the public account on April 27th.
2. The tweet of the Skin Care English Class was published on the public account on April 29th.
3. A meeting was held on April 30th.

Week 18

Project

1. The topic and content of the lecture was discussed on May 2nd.
2. "The Synthetic Biology Popular Science" was posted on the public account on May 6th.
3. We discussed the topic of the questionnaire on May 8th.

HP

1.We went to Winkey Pharmaceutical R&D Co., Ltd. for an interview on May 2nd.

Week 19

Project

1. We had the second meeting with SZU and SUSTech on May 10th. At the meeting, students from SUSTech put forward the requirements for the declaration structure and declaration title.
2. The project interpretation was posted on the public account on May 11th.
3. The first tweet of "reader" (audiobook) was posted on the public account on May 12th.
4. "Skin Care English Class" was posted on the public account on May 13th.
5. Carried out the project report of each group's winter vacation project offline on May 15th.

Week 20

Project

1. The second tweet of ""reader"" was posted on the public account on May 16th.
2. The students of the EXP group went to the Shenzhen Institute of Advanced Technology, Chinese Academy of Csiences for training on Laboratory Safety Regulations on May 18th.
3. The third tweet of "reader" was posted on the public account on May 19th.
4. "No distance with synthetic biology" was posted on the public account on May 20th.

HP

1. Interviewed cosmetic consumers on May 18th.

Week 21 - 30

Week 21

Project

1. The fourth tweet of "reader" was posted on the public account on May 23rd.
2. The fifth tweet of "reader" was posted on the public account on May 26th.
3. "English class" was posted on the public account on May 27th.
4. An offline meeting was held on May 29th.

HP

1. Interviewed dermatologists Jingkai Huang and Jun Huang, plastic surgeons Yuru Shang at Southern University of Science and Technology hospital on May 24th as well as interviewed Zhijun Tan of a plastic surgeon at Shenzhen University General hospital.

Week 22

Project

1. The students of the EXP group went to Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences for safety training and laboratory safety examination on June 1st.
2. Participated in the Southern China iGEM meetup on June 3rd.
3. Held an offline meeting on June 5th.

Week 23

Project

1. The eighth "reader" was posted on the public account on June 6th.
2. An offline meeting was held on June 12th.
3. The ninth tweet of "reader" was posted on the public account on June 9th.

HP

1. We cooperated with the Japanese Club of our school, saw the film Escape from Clone Island, which is about synthetic biology to the members online to promote synthetic biology.

Week 24

Project

1. The tenth tweet of "reader" was posted on the public account on June 13th.
2. The eleventh tweet of "reader" was posted on the public account on June 16th.
3. We watched the seminar online on the topic of "Innovative Application of Synthetic Biology in the Field of New Materials for Personal Care" on June 17th.
4. "Chinese version of synthetic biology" was posted on our public account on June 17th.

HP

1. Conducted a popular science activity of synthetic biology with members of Inspiration Creativity Club online.
2. interviewed Dr. Mingxing Tang on June 16th.

Week 25

Project

1. The twelfth tweet of "reader" was posted on the public account on June 20th.
2. The thirteenth tweet of "reader" was posted on the public account on June 23rd.
3. The EXP group reported and summarized their experiment on June 26th.

HP

1. Held an activity in Nanshui Kindergarten to on June 22nd.
2. Reinterviewed Dr. Mingxing Tang on June 25th.

Week 26

Project

1. The fourteenth of "reader" was posted on the public account on June 27th.
2. The eighth of project interpretation was posted on the public account on June 29th.
3. The fifteenth tweet of "reader" was posted on the public account on June 30th.
4. "English class" was posted on the public account on July 1st.

Week 27

Project

1. The sixteenth tweet of "reader" was posted on the public account on July 4th.
2. Summarized last week's problems and assigned tasks in an online meeting on July 6th.
3. Posted the seventeenth of "reader" tweets on the public account.
4. Held an online meetup with NAU-China,NJTech_China,UCAS-China,UCAC-China,HBUT-China on July 8th.
5. Participated in the "2022 Synthetic Biology Competition-Innovation Competition".

Week 28

Project

1. Posted the eighteenth tweet of "reader" on the public account on July 11st.
2. A questionnaire was posted on July 13th.
3. The first meeting was held with BNUZH-China on July 14th.
4. The first meeting with HBUT-China was held on July 14th.
5. A tweet about skin care was posted on the public account on July 14th.
6. Posted a tweet about participating in the "Synthetic Biology Competition-Innovation Competition" on the official account on July 15th.
7. Participated in the 2022 China iGEM Online Meetup hosted by GXU-China, Jilin China 2022, Jiangnan_China, and NPU_CHINA on July 16th
8. The first meeting with NEFU_China was held on July 17th.

Lab

1. The J23102-RBS003422-gshF-T0-pSEVA331(JRgTp) plasmid was constructed and transformed into E. coli TOP10.
2. Built the plasmids about the lysis and safety module.

Week 29

Project

1. The project interpretation series tweets were posted on the public account on July 18th.
2. Held an online meeting with NWU-CHINA-A on July 19th.
3. A summary tweet was posted on the public account on July 19th.
4. "skin care class" was posted on the public account on July 20th.
5. "English class" was posted on the public account on July 23rd.
6. Participated in the 4th Northwestern China iGEM Meetup hosted by NWU-CHINA-A on July 23rd.
7. Summarized the HP work we've done as well as the feedback from the questionnaire on July 24th.

Lab

1. Verified the experssion of GshF, and used The GSH and GSSG Assay Kit of Beyotime to test the production of GSH in E. coli TOP10.

Week 30

Project

1. Held an online meeting on July 26th.
2. Poseted "Skin care class" was posted on the public account on July 27th.
3. Held an online meeting on July 27th.
4. The third online meeting was held with HBUT-China on July 29th.
5. Had an online meeting On July 29th.
6. "English class" was posted on the public account on July 29th.
7. Held an online meeting with the BNUZH team on July 31st.

HP

1. An activity called "A Midsummer 366 Appointment" was held.
2. The offline event "A Midsummer 366 Covenant" was held on July 31st.

Lab

1. Shook strains that verified to be correct ,and extracted plasmid as well as transferred into G. hansenii ATCC53582 with electrotransformation.
2. Added LVA hydrolysis label on strains that grew in dark and didn't grow in light.
3. Conducted the preliminary exploration of emulsion.

Week 31 - 40

Week 31

Project

1. "English class" was posted on the public account on August 5th.
2. Had an online communication with BNUZH-China at the Tencent Conference on August 7th.

HP

1. Visited the Yakult factory with the CUHKSZ team and non-competition members on August 3rd.
2. Held a lecture named "Enter the World of Synthetic Biology", which was participated by nearly 300 college students.

Lab

1. Verified whether J23102-RBS003422-gshF-T0-pSEVA331(JRgTp) plasmid exist into G. hansenii ATCC53582.
2. Used The GSH and GSSG Assay Kit of Beyotime to test the production of GSH in G. hansenii ATCC53582.
3. Optimised blue light responsive system of strains that lysedwell last week: LVA hydrolysis label was knocked out.
4. Determined the exploration conditions of live bacterial emulsion/gel.

Week 32

Project

1. "Skin care class" was posted on our public account on August 10th.
2. Conducted an online interview with Lifegen Co., Ltd on August 10th.
3. Had an online meeting with the HBUT-China at the Tencent Conference on August 11th.
4. Held an online meeting on August 11th.
5. "English class" was posted on our public account on August 12th.
6. Had an online meeting with the NWU team at the Tencent conference on August 12th.
7. Had an online meeting with the BNUZH at the Tencent conference on August 14th.

HP

1. Conducted an activity in Wulonggang Village on August 12th.
2. Went to Guangzhou Question Mark Art Institute to conduct science popularization of synthetic biology on August 15th.
3. Interviewed the experimenters of Bode Chuangyan (Guangzhou) Biotechnology Co., Ltd. on August 14th.

Lab

1. Plotted growth curve of G. hansenii ATCC53582 which contains J23102-RBS003422-gshF-T0-pSEVA331(JRgTp) plasmid.
2. Repeated emulsion experiment.

Week 33

Project

1. Participated in the meeting of CCiC.
2. "English class" was posted on our public account on August 19th.

HP

1. Interviewed Ms.Lee, a skin health practitioner at Naturade Beauty Salon on August 22nd.

Lab

1. Plotted the growth curve of G. hansenii ATCC 53582 that containes J23102-B0034-gshF-pSEVA331 plasimds at different concentrations of chloramphenicol.
2. Extracted plasimds that lysed well in the E. coli and transferred into G. hansenii ATCC53582 with electrotransformation.
3. Explored the conditions of live bacterial gel.

Week 34

Project

1. We had an online meeting with HBUT-China at the Tencent Conference on August 25th.
2. We had an regular meeting on August 26th.
3. We had an online meeting with BNUZH-China at the Tencent Conference on August 28th.
4. We had an weekly meeting on August 28th.

HP

1. Went to Liyayuan West Community for science popularization on August 23rd.

Lab

1. Plaques of G. hansenii ATCC53582 that contains lysis gene were pointed two plates: some plates put on dark place, others put on light place.
2. Determined the condition of live bacterial gel.

Week 35

Project

1. We had an online meeting with NWU-CHINA-A at the Tencent Conference on September 2nd.
2. We had an online meeting with CUHKSZ at the Tencent Conference on September 3rd.
3. We had an weekly meeting on September 4th.

Week 36

Project

1. We had an offline meeting on September 9th.
2. We had an online meeting with BNUZH-China at the Tencent Conference on September 11th.

HP

1. We presented our project and synthetic biology to freshmen on September 5th.
2. We brought the abiotics students into the lab to experience the product form of our project on September 10th.

Week 37

Project

1. We had an weekly meeting on September 13th.

HP

1. We took the school's minority students into the laboratory on September 17th.

Week 38

Project

1. Edited wiki pages.
2. Prepared the final presentation.

Week 39

Project

1. Edited wiki pages.
2. Prepared the final presentation.

Week 40

Project

1. Edited wiki pages.
2. Prepared the final presentation.

HP

1. We performed Chinese shadow puppetry on the spotground on October 6th.
2. Held the activity of biological Chinese paper-cutting on October 8th.