Previous Basic Parts:

BioBrick ID Name Type Length Description
BBa_J23100 Constitutive Promoter Regulatory 35 bp DNA sequence that can initiate transcription of the downstream DNA sequence
BBa_J23118 Constitutive Promoter Regulatory 35 bp DNA sequence that can recruit transcriptional machinery and lead to transcription of the downstream DNA sequence
BBa_K896008 zinTp (Cd2+ sensing promoter) Regulatory 81 bp The metal sensing promoter expresses the downstream gene only in the presence of cadmium ions.
BBa_K342003 OmpR234 protein, with higher effect on Curli promoter Regulatory 739 bp Upregulation of OmpR234, activates curli csgD promoter.
BBa_B0034 Strong RBS RBS 12 bp RNA sequence found in mRNA to which ribosomes can bind and initiate translation.
BBa_K805015 CsgD,the master regulator of biofilm formation Coding 651 bp CsgD is the major regulator of biofilm formation as it helps the synthesis of curli fimbriae.
BBa_K103006 OmpA outer membrane protein A fused to linker Tag 464 bp Proteins fused to the OmpA link are displayed on the cell surface.
BBa_B0015 Double terminator Terminator 129 bp It consists of both B0010 and B0012 terminators. It occurs at the end of a gene or operon and causes transcription to stop.

New Basic Part:

BioBrick ID Name Type Length Description
BBa_K4509369 Acid phosphatase (aphA) Coding 714 bp To express acid phosphatase enzymes.

New Composite Parts:

BioBrick ID Name Type Length Description
BBa_K4509469 csgD and OmpR234 Dual Expression Composite 1616 bp By overexpressing both the proteins, curli fibre production can be increased.
BBa_K4509669 Cell surface aphA with Cd2+ sensing promoter Composite 2122 bp Acid phosphatase is expressed as a cell surface protein in the presence of cadmium.
BBa_K4509769 Cell surface aphA with constitutive promoter Composite 2076 bp Acid phosphatase is expressed as a cell surface protein.