All the parts listed in our collection have common attributes in modification. All 5 composite parts have promoter parts added to amplify the expression of the enzymes such as Acid phosphatase and HorseRadish Peroxidase. BBa_K4509669 and BBa_4509769 possess a metal sensitive and a constitutive promoter respectively. The constitutive promoter reduces the burden of the cell as well. Following this, we have BBa_4509569, BBa_4509869 and BBa_4509420, each of which contains different constitutive promoters that increase the expression of the enzymes while reducing cell burden.
| BioBrick ID | Name | Type | Length | Description |
|---|---|---|---|---|
| BBa_K4509669 | Cell surface aphA with Cd2+ sensing promote | Composite with metal sensing promoter | 2122 bp | Increases the expression of Acid phosphatase in the presence of Cd2+ |
| BBa_K4509769 | Cell surface aphA with constitutive promoter | Composite with constitutive promoter | 2076 bp | Increases the cell surface expression of Acid phosphatase. |
| BBa_K4509569 | HorseRadish Peroxidase with constitutive promoter J23100 | Composite with constitutive promoter | 1120 bp | Increase in HRP production. |
| BioBrick ID | Name | Type | Length | Description |
|---|---|---|---|---|
| BBa_K4509469 | csgD and OmpR234 Dual Expression | Composite with constitutive promoter | 1616 bp | Acid phosphatase production with reduced cell burden. |
| BBa_K4509869 | HorseRadish Peroxidase with constitutive promoter J23106 | Composite with constitutive promoter | 1120 bp | HRP production with reduced cell burden. |
| BBa_K4509420 | HorseRadish Peroxidase with constitutive promoter J23116 | Composite with constitutive promoter. | 1120 bp | HRP production with reduced cell burden. |