Introduction to iGEM

Team recruitment began

Interviews were conducted for students who showed interest in joining iGEM.

The whole iGEM team was finalised.

First official offline meet-up was held.

Lectures from faculties on iGEM protocols

1st brainstorming session

Literature review was done.

Had a 1st online meet-up with the seniors for getting to know about iGEM and their experience.

Opened the iGEM lab and had the stock check done.

Brainstorming session.

Literature review was done

Official group picture was taken.

Fumigation of lab.

1st lab discussion.

Literature review was done.

Stock check

Brainstorming session

Troubleshoots

Literature review

Advice from investigators

Discussion with instructors

Stock check

Safety Check

Registration of the students into the iGEM REC Chennai Team.

Accepted as member of REC-CHENNAI iGEM team.

Arrival of E.coli Alpha strain.

Grant submission

Subculture.
Safety forms submitted.

iGEM kit received

iGEM kit unboxed

Subculture.

Submitted safety and security grant.

Subculture, online meet for team discussion.

Subculture and testing viability of mother culture and discussion about the biochemical assay.

Online meet.

Subculture and fumigation of lab.

Offline meetup for work discussion.

Subculture

DH5 alpha test and reactor building discussions.

Subculture and confirmation tests

Online meet discussion for AIIMS.

AIIMS presentation

Collecting K12 and DH5 alpha strain from IITM.

Madras University meet with Dr. B. Anandan

SBOA School and Junior College HP, Online meet with Team Waterloo.

Subculture and stock check

Online meet to discuss for sponsorship possiblities & college level event

Meet with our advisor Mr. Harikumar, discussion with Dr. P. Saravanan. Online meet with Madlyn Thompson.

Night stay for lab work: Biofilm assay and Subculture

Sacred Heart School HP

Rehersal for game launch program.

CURCOLI game launch and subculture.

Sponsorship meet with Textan MD.

Online meet with Mr. Vijay Bhoopalan.

Bhavan's Rajaji Vidyashram School HP

Subculture and Biofilm new protocol attempt

Successfully biofilms formed

Discussion for beach HP and subculture

Kalpakkam Scientist meet with Dr. Venkat Nancharaiah and Dr. Kiran

Protocol Optimization: MTT assay, pNPP assay and subculture

Edward Elliots Beach HP

Meet with iGEM-Munich

Protocol optimization: Growth curve, Cd toxicity, subculture and stock check

Promotion video uploaded

Meet with Twist Bioscience

Placed order in Twist Bioscience

Night stay for Protocol Optimization: Transformation, HRP assay and subculture

Primers synthesis order

Final Safety Form submitted

Online meet with Advisors

Meet with IIT Delhi and subculture

Received primers

Night stay for Protocol Optimization: Transformation, Protein Quantitative assays.

Meet with Mr. Deepak Kumar

Received parts from Twist bioscience and subculture

Lab Activity: PCR and analysis of plasmid.

Lab Activity: Restriction Digestion, Ligation and Plasmid mini prep.

Lab Activity: Transformation.

Lab Activity: Isolation of Colonies and subculture.

Reactor setup

Biofilm assay

Characterization: CV, Congo red, Saffranin

Characterization: Growth Curve, Cd toxicity assay and subculture

Characterization: Samples given for AAS assay and SEM, MTT assay

Characterization: MTT assay, pNPP assay

Characterization: HRP assay

Final wiki edition

Final wiki edition, Wiki, Registry, Judging form freeze

Team meet

Poster and PPT Making

Poster and PPT Making

Presentation preparation

Team meet

Grand Jamboree

Grand Jamboree

Grand Jamboree