Introduction to iGEM
Team recruitment began
Interviews were conducted for students who showed interest in joining iGEM.
The whole iGEM team was finalised.
First official offline meet-up was held.
Lectures from faculties on iGEM protocols
1st brainstorming session
Literature review was done.
Had a 1st online meet-up with the seniors for getting to know about iGEM and their experience.
Opened the iGEM lab and had the stock check done.
Brainstorming session.
Literature review was done
Official group picture was taken.
Fumigation of lab.
1st lab discussion.
Literature review was done.
Stock check
Brainstorming session
Troubleshoots
Literature review
Advice from investigators
Discussion with instructors
Stock check
Safety Check
Registration of the students into the iGEM REC Chennai Team.
Accepted as member of REC-CHENNAI iGEM team.
Arrival of E.coli Alpha strain.
Grant submission
Subculture.
Safety forms submitted.
iGEM kit received
iGEM kit unboxed
Subculture.
Submitted safety and security grant.
Subculture, online meet for team discussion.
Subculture and testing viability of mother culture and discussion about the biochemical assay.
Online meet.
Subculture and fumigation of lab.
Offline meetup for work discussion.
Subculture
DH5 alpha test and reactor building discussions.
Subculture and confirmation tests
Online meet discussion for AIIMS.
AIIMS presentation
Collecting K12 and DH5 alpha strain from IITM.
Madras University meet with Dr. B. Anandan
SBOA School and Junior College HP, Online meet with Team Waterloo.
Subculture and stock check
Online meet to discuss for sponsorship possiblities & college level event
Meet with our advisor Mr. Harikumar, discussion with Dr. P. Saravanan. Online meet with Madlyn Thompson.
Night stay for lab work: Biofilm assay and Subculture
Sacred Heart School HP
Rehersal for game launch program.
CURCOLI game launch and subculture.
Sponsorship meet with Textan MD.
Online meet with Mr. Vijay Bhoopalan.
Bhavan's Rajaji Vidyashram School HP
Subculture and Biofilm new protocol attempt
Successfully biofilms formed
Discussion for beach HP and subculture
Kalpakkam Scientist meet with Dr. Venkat Nancharaiah and Dr. Kiran
Protocol Optimization: MTT assay, pNPP assay and subculture
Edward Elliots Beach HP
Meet with iGEM-Munich
Protocol optimization: Growth curve, Cd toxicity, subculture and stock check
Promotion video uploaded
Meet with Twist Bioscience
Placed order in Twist Bioscience
Night stay for Protocol Optimization: Transformation, HRP assay and subculture
Primers synthesis order
Final Safety Form submitted
Online meet with Advisors
Meet with IIT Delhi and subculture
Received primers
Night stay for Protocol Optimization: Transformation, Protein Quantitative assays.
Meet with Mr. Deepak Kumar
Received parts from Twist bioscience and subculture
Lab Activity: PCR and analysis of plasmid.
Lab Activity: Restriction Digestion, Ligation and Plasmid mini prep.
Lab Activity: Transformation.
Lab Activity: Isolation of Colonies and subculture.
Reactor setup
Biofilm assay
Characterization: CV, Congo red, Saffranin
Characterization: Growth Curve, Cd toxicity assay and subculture
Characterization: Samples given for AAS assay and SEM, MTT assay
Characterization: MTT assay, pNPP assay
Characterization: HRP assay
Final wiki edition
Final wiki edition, Wiki, Registry, Judging form freeze
Team meet
Poster and PPT Making
Poster and PPT Making
Presentation preparation
Team meet
Grand Jamboree
Grand Jamboree
Grand Jamboree