Team:OUC-China

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This year,we use the following protocols to complete our experiments.

Part 1 Transporter RNA Construction and Testing

PCR

Construction of RNA library (using T7 transcription kit)

SELEX

RNA Antisense Purification

Assembly of Transporting RNA

Aptamer Kd measurement

In vitro transport efficiency and rate measurement

Part 2 Quorum Sensing

Preparation of electrocompetent cells

Preparation of 2-IP solution

Determination of growth curve and background expression intensity of SSRE promoter of Aureobasidium melanogenum P16

Determination of dynamic regulatory range of SSRE promoter

Part 3 Kill Switch

RNA SELEX

Functional Verification of Riboswitch

Part 4 Other experiments

Agarose Gel Electrophoresis