Our team created a total of 15 parts in the iGEM registry. These parts included sequences we ordered from AddGene and sequences we engineered through Gibson Cloning and polymerase chain reactions.
| Name | Type | Description | Designers | Length |
|---|---|---|---|---|
| BBa_K4482000 | Coding | Gene coding for the FGFR2 receptor, a member of the FGFR (fibroblast growth factor receptor) family. | Sourced from AddGene (catalog 116741) | 2466 |
| BBa_K4482002 | Coding | Gene coding for the NTRK3 receptor, a member of the NTRK (neurotrophic tyrosine receptor kinase) family. | Sourced from AddGene (catalog 20551) | 1836 |
| BBa_K4482003 | Coding | Gene coding for the human insulin receptor, a transmembrane protein expressed on epithelial cells that binds to insulin. | Sourced from AddGene (catalog 22286) | 4146 |
| BBa_K4482004 | Coding | Gene coding for FGFR2 (BBa_K4482000) fused to the sequence for eGFP. | Mustafo Mustafokulov, Hailey Seltzer, Kohana Henson, Marco Giordano, Maya Giordano, Vanessa Chen | 3183 |
| BBa_K4482005 | Coding | Gene coding for NTRK3 (BBa_K4482002) fused to the sequence for eGFP. | Mustafo Mustafokulov, Hailey Seltzer, Kohana Henson, Marco Giordano, Maya Giordano, Vanessa Chen | 2553 |
| BBa_K4482006 | Coding | Gene coding for the heavy chain of HIRMAb (human insulin receptor monoclonal antibody). | Matteo Da Ros, Mustafo Mustafokulov | 1332 |
| BBa_K4482007 | Coding | Gene coding for the light chain of HIRMAb (human insulin receptor monoclonal antibody). | Matteo Da Ros, Mustafo Mustafokulov | 654 |
| BBa_K4482008 | Coding | Gene coding for FGF-2 (fibroblast growth factor 2) protein. | Matteo Da Ros | 468 |
| BBa_K4482010 | Coding | Gene coding for NT-3 (neurotrophin 3) protein. | Matteo Da Ros | 813 |
| BBa_K4482011 | Composite | Sequence for HIRMAb's heavy chain (BBa_K4482006) fused to FGF-2 (BBa_K4482008). | Mustafo Mustafokulov, Hailey Seltzer, Kohana Henson, Marco Giordano, Maya Giordano | 1800 |
| BBa_K4482012 | Composite | Sequence for HIRMAb's heavy chain (BBa_K4482006) fused to NT-3 (BBa_K4482010). | Mustafo Mustafokulov, Hailey Seltzer, Kohana Henson, Marco Giordano, Maya Giordano | 2145 |
| BBa_K4482013 | Coding | Gene coding for HIR (BBa_K4482003) fused to the sequence for eGFP. | Mustafo Mustafokulov, Hailey Seltzer, Kohana Henson, Marco Giordano, Maya Giordano | 4896 |
| BBa_K4482014 | Coding | Sequence for Human Insulin Receptor Monoclonal Antibody (HIRMAb) | Mustafo Mustafokulov, Hailey Seltzer, Kohana Henson, Marco Giordano, Maya Giordano | 3732 |
| BBa_K4482015 | Coding | Sequence for HIRMAb (BBa_K4482014) fused to FGF-2 (BBa_K4482010). | Mustafo Mustafokulov, Hailey Seltzer, Kohana Henson, Marco Giordano, Maya Giordano | 4197 |
| BBa_K4482016 | Coding | Sequence for HIRMAb (BBa_K4482014) fused to NT-3 (BBa_K4482010). | Mustafo Mustafokulov, Hailey Seltzer, Kohana Henson, Marco Giordano, Maya Giordano | 4542 |
These plasmids contain the protein-coding sequences for HIR, FGFR2 and NTRK3 attached to eGFP. The expression of HIR-eGFP, FGFR2-EGFP and NTRK3-EGFP is under control of the CMV promoter, which allows for the expression of the plasmid in mammalian cells. eGFP and HIR/FGFR2/NTRK3 are connected by a flexible linker that allows for independent folding into their expected 3D conformation.
These plasmids contain the sequences for HIRMAb separately attached to the sequences for FGF-2 and NT-3. FGF-2 and NT-3 were fused to the C-terminus of HIRMAb's heavy chain so that they could be transported from the blood stream through the BBB into the brain once HIRMAb binded to the endothelial cell's insulin receptor. Once in the brain, the proteins would bind to their cell receptors on neurons, promoting neuroprotective benefits.
