Overview

1. Preparation of LB-agar plates with selection antibiotic
2. High efficiency transformation protocol
3. Plasmid production and purification using HiSpeed Plasmid Midi Kit
4. QIAquick PCR Purification Kit
5. QIAquick Gel Extraction Kit
6. QIAprep Spin Miniprep Kit
7. High Efficiency Transformation Protocol
8. NEBuilder HiFi DNA Assembly Cloning
9. Polymerase Chain Reaction
10. Transfection of CHO cells with jetOPTIMUS
11. Testing protein-receptor interaction for Trojan horses
12. Transwell experiment

Preparation of LB-agar plates with selection antibiotic

High efficiency transformation protocol

Plasmid production and purification using HiSpeed Plasmid Midi Kit

QIAquick PCR Purification Kit

QIAquick Gel Extraction Kit

QIAprep Spin Miniprep Kit

High Efficiency Transformation Protocol

NEBuilder HiFi DNA Assembly Cloning

Polymerase Chain Reaction

Transfection of CHO cells with jetOPTIMUS

Testing protein-receptor interaction for Trojan horses

Transwell experiment