eGFP (BBa_K1911005)

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  We added information regarding the use of this part as a marker for the identification of cells positively transfected with our proteins of interest. In order to verify that the Trojan horses we designed for our project are still able to interact with their specific receptor in the brain, we designed fusion proteins made of the HIRMAb, FGF-2, and NT-3 receptors marked with eGFP at their C-terminus (the cytoplasmic side of the receptor). The presence of eGFP will allow us to identify cells expressing the recombinant receptor and verify the positive interaction of the Trojan horse with the receptor while accounting for non-specific interaction of the Trojan horse with the cell surface in cells not expressing the receptor.

Insulin Receptor (BBa_K3237013)

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  We updated the information regarding the insulin receptor part by adding the description of how we used this part for our project. The Trojan horses we designed take advantage of the active role that the insulin receptor has in the receptor-mediated transport of big molecules from the bloodstream through the Blood-Brain Barrier (BBB) into the brain. These Trojan horses are formed by a neuroactive protein fused to the C-terminus of the heavy chain of a recombinant antibody specific for the human insulin receptor (HIRMAb). HIRMAb binds to the insulin receptor without interfering with the binding of its natural ligand insulin. When the insulin receptor transports insulin through the BBB it carries the HIRMAb along with it.