·The name of the Laboratory is Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medical
·Relying on Nanjing University of Chinese Medicine
·Address: 138 Xianlin Road, Nanjing City, Jiangsu Province (Postcode: 210023)

·When entering the laboratory, it is necessary to read the laboratory regulations carefully and fully understand the risks of the experiment and related reagents
·Wear a lab coat, gloves, and a mask.
·Wear long pants and not show your ankles
·Discard the waste into the waste liquid tank and waste liquid bucket, do not throw it away randomly
·Procedure for entering the cell room: close the door -- take off the coat -- use the lab coat and change the slippers -- shower in the air -- wear the lab coat of the cell room and close the door after you leave
·It is forbidden to carry electronic equipment that has a signal conflict with the equipment and instruments in the laboratory
·It is forbidden to eat and drink in the laboratory
·No noise and play in the laboratory
·Put out your lab coat, gloves, and mask before leaving the lab
·All articles in the laboratory must not be taken out of the laboratory
·After the end of the experiment, it is necessary to clean the table and instrument, empty the waste liquid tank, and return the pipette to the measuring range. Share the actual consumables and put them back in place. Don't keep personal effects

--Centrifuge:
Glass centrifuge tubes cannot be used in high and over-speed centrifuges
·Clean the centrifuge chamber and head regularly, and dry the condensed water in the chamber
·Balance the sample before centrifugation
·After use, open the door until the chamber restores to normal temperature, and disinfect and sterilize
-- Constant temperature culture shaker:
·Ensure that the indoor temperature ranges from 18°C to 34 °C, and the relative humidity is as high as 90%
·The equipment is properly leveled by placing a bubble level on the drive bearing housing at the top of the center
·In the process of sampling, if the sample is found to be missing (including the drop of the plug), the main power should be turned off, and the debris should be removed with tweezers as far as possible, and disassembled if necessary!
·When removing the sample, turn off the power or set the speed to zero.
·The surface of the instrument should be scrubbed frequently to keep it clean, and the side of the instrument control box should be avoided when scrubbed to avoid water droplets into the instrument and damaging the instrument
-- Electrophoresis apparatus:
·After the electrophoresis apparatus is energized, do not touch the parts that may be charged, do not get the electrophoresis tank to remove things,
·Electrophoresis with different media supports should not be performed simultaneously.
·When the total current does not exceed the rated current of the instrument (maximum current range), it can be used in multi-slot association.
-- Pipette：
·First of all, choose the pipette that suits the measuring range of your own experiment
·Check whether it is at maximum value before use
·After the inspection, turn the upper knob to the used value, and pay attention to the nozzle should be vertical suction during use to avoid contamination
·Remember to screw it back to the maximum value when you're done

·In our experiment, Escherichia coli M15 and Ins-832/13 were used in the study which were non-toxic and non-pathogenic, all experiments were carried out on a sterile experimental bench without external contamination. And under the guidance of a teacher.
During the experiment, all of the apparatuses, such as Petri dishes, beakers, and measuring cylinders, are cleaned or sterilized before use. All wasted liquid from culturing cells has been properly disposed of and all medical wastes generated in experiments are stored in designated areas, which minimizes the risk of biohazardous substances in the laboratory polluting the environment

·In the lab, we don't use any animal or human genes.
·The purpose of all our experiments and activities is scientific research