Nowadays, there are a large number of patients with type 2 diabetes in the world, and the discovery of new anti-type 2 diabetes drugs is imminent. Glucokinase (GK) is distributed in the pancreas, liver, brain and intestine, and has the effect of regulating blood sugar balance. In this project, we will construct a prokaryotic expression plasmid of human GK, purify the GK protein, establish an in vitro human GK activity detection platform, screen for drugs with GK agonistic activity, and test them at the cellular level to enhance glucose-stimulating insulin secretion and protect pancreatic islets, so as to contribute to the treatment of type 2 diabetes.