The following list provides you with an overview of all the parts we have used in our project. Parts directly obtained from the BioBrick Registry are listed below ("Existing Parts"). Moreover, four new parts were added to the registry among them our codon-optimized genes encoding the glyphosate-degrading enzymes (goxA, goxB & C-P-Lyase). No existing parts were improved further.
| Part Registry ID | Part Name | Description | Part Category | Compatibility |
|---|---|---|---|---|
| BBa_K090505 | Ribosome Binding Site (RBS) | Bacillus subtilis consensus RBS | Basic | BioBrick Type IIS |
| BBa_K780000 | Terminator | Terminator for Bacillus subtilis | Basic | BioBrick Type IIS |
| BBa_K143012 | Promoter | Constitutive promoter (Pveg) for Bacillus subtilis | Basic | BioBrick Type IIS |
| BBa_K143015 | Promoter | IPTG inducible promoter for Bacillus subtilis | Basic | BioBrick Type IIS |
| BBa_K2292207 | Promoter | Stationary phase promoter (Pylb) for Bacillus subtilis | Basic | BioBrick Type IIS |
| Part Registry ID | Part Name | Description | Part Category | Compatibility |
|---|---|---|---|---|
| BBa_K4373001 | DNA sequence | goxB encoding for glyphosate dehydrogenase | Basic | Type IIS |
| BBa_K4373002 | DNA sequence | goxA encoding for glyphosate dehydrogenase | Basic | Not compatible |
| BBa_K4373003 | DNA sequence | Gene encoding multimeric enzyme complex domain C-P-Lyase | Basic | Type IIS |
| BBa_K4373005 | 5’UTR | 5’UTR including RBS | Composite | BioBrick Type IIS |