Here, you can find an overview of all the protocols we have used for our experimental part. We would like to thank Johan Bonde and Claes van Wachenfeldt in particular, who were kindly enough to provide us with some of there optimized protocols for cloning in E. coli and transformation into Bacillus subtilis, subsequently. @ Future iGEMers - Feel free to use these for your own projects. And Good Luck.