In this competition season, our team made 4 new parts, which are involved in the development of a detector used for major depressive disorder (MDD) diagnoses. Each of them comprises toehold switch, linker sequence and marker protein or enzyme, which are described as follows:
BBa_K4167000:
It comprises the antisense sequence of miRNA 34a-5p, RBS, Linker and part sequence of miRNA 34a-5p, which form a toehold switch, as well as the gene of marker protein amilCP. At the presence of miRNA 34a-5p, it binds to its antisense sequence, opening the toehold switch to trigger the expression of amilCP, which is easily measured. It is used to detect the amount of miRNA 34a-5p in samples.
BBa_K4167001:
It comprises the antisense sequence of miRNA 221-3p, RBS, Linker and part sequence of miRNA 221-3p, which form a toehold switch, as well as the gene of marker protein mRFP. At the presence of miRNA 221-3p, it binds to its antisense sequence, opening the toehold switch to trigger the expression of mRFP, which is easily measured. It is used to detect the amount of miRNA221-3p in samples.
BBa_K4167002:
It comprises the antisense sequence of miRNA let-7d-3p, RBS, Linker and part sequence of miRNA let-7d-3p, which form a toehold switch, as well as the gene of marker protein amilGFP. At the presence of miRNA let-7d-3p, it binds to its antisense sequence, opening the toehold switch to trigger the expression of amilGFP, which is easily measured. It is used to detect the amount of miRNA let-7d-3p in samples.
BBa_K4167666:
It comprises the antisense sequence of miRNA 34a-5p, RBS, Linker and part sequence of miRNA 34a-5p, which form a toehold switch, as well as the LacZ gene of β-galactosidase. At the presence of miRNA 34a-5p, it binds to its antisense sequence, opening the toehold switch to trigger the expression of β-galactosidase which catalyzes the substrate X-gal to produce 5-bromo-4-chloro Indigo (blue color). It is used to detect the amount of miRNA 34a-5p in samples.
Our new parts are summarized in this table:
| Name | Type | Description | Designers | Length |
|---|---|---|---|---|
| BBa_K4167000 | coding AmilCPr | Toehold switch-amilCP triggered by miRNA 34a-5p | Xinzichu Xie | 750bp |
| BBa_K4167001 | coding mRFP | Toehold switch-mRFP triggered by miRNA 221-3p | Xinzichu Xie | 762bp |
| BBa_K4167002 | coding AmilGFP | Toehold switch-amilGFP triggered by miRNA let-7d-3p | Xinzichu Xie | 780bp |
| BBa_K4167666 | coding LacZ | Toehold switch-LacZ triggered by miRNA 34a-5p | Lin Wange | 3156bp |