| ACT1p-scFv-GFP-actin(BBa_K4203000)
| Fused Protein for expression of ACT1 of Saccharomyces cerevisiae 288c |
After codon optimization, the fusion protein Act1P-SCFV-GFP-actin was planing to express in Saccharomyces cerevisiae BY4741. It was linked to the vector pESC-URAby adding BamH I and Sac I restriction sites at the 5 'and 3' ends, respectively. 4848-5459 bp represents the ACT1 promoter,4107-4847 bp represents scFv component,4062-4106 bp represents (GGGGS) Χ3 linker,3351-4061 represents GFP, 3321 -3350 bp represents (GS) Χ5 Linker, 2187-3320 bp represents S288C ACT1 gene.(+/-) |
Yixuan Wang; Zongjun Lee |
9149 bp |
| TDH3p-DmIav-sunTag-BFP(BBa_K4203001)/a>
| Fused Protein for expression of DmIav of Drosophila melanogaster |
After codon optimization, the fusion protein TDH3p-DmIav-sunTag-BFP was planingto express in Saccharomyces cerevisiae BY4741. It was linked to the vector pESC-LEU by adding Sac I and Xho I restriction sites at the 5 'and 3' ends, respectively. 3352-4018 bp represents the TDH3 promoter, 4025-7393 bp represents DmIav component, 7394-7423 bp represents (GS) Χ5 linker, 7424-8137 bp represents sunTag, 8138-8194 bp represents P2A Linker, 8207-8914 bp represents TagBFP. |
Yixuan Wang; Zongjun Lee |
12534 bp |
| ADH1p-DmNan-P2A-mCherry(BBa_K4203002)
| Fused Protein for expression of DmNan of Drosophila melanogaster |
After codon optimization, the fusion protein ADH1p-DmNan-P2A-mCherry wasplaning to express in Saccharomyces cerevisiae BY4741. It was linked to the vector pESC-HIS by adding BamH I and Sac I restriction sites at the 5 'and 3' ends, respectively. 5564-5960 bp represents the ADH1 promoter, 3059-5557 bp part represents DmNan component, 2993-3094 bp represents P2A linker, 2264-2980 bp represents mCherry. |
Yixuan Wang ;Zongjun Lee |
9655 bp |
