Plasmid design




"Each cell can produce its own unique sound."


Our project is dedicated to the research and deepening of sound, which comes from a report on "Cellular Acoustics" we saw at the beginning of the project. If all cells beat like heart cells, is the vibration enough to produce a detectable sound? Can we make our bacteria sense this sound? Is such research useful? We look forward to tracking the clues of cellular acoustics. Fortunately, we found this information from the data:
"Each cell can produce its own unique sound."
"Kinesin is connected to the cytoskeleton (responsible for maintaining the integrity and shape of the cell structure), and the cytoskeleton is connected to the cell membrane." Therefore, the movement of motion proteins is considered to be transmitted to the cell membrane through the cytoskeleton, causing the cell membrane to oscillate. " "The unified movement of intracellular motor proteins is believed to be the reason behind the cell voice."
These data support our view to some extent. As we all know, the most interesting application in the field of cellular acoustics is the detection of cancer and other diseases. We imagine that the existence of cancer cells can be tested by the cells' voice or the perception of this special sound. Therefore, the first development direction of our project is to make cells use sound to perceive cancer through operation.
Hearing is the ability of animals to perceive sound and the process of transforming mechanical energy into electromagnetic signals. However, its mechanism is not completely clear, and whether there is a similar sensory system in microorganisms has not been studied. BJWZ China is ready to try to set a precedent. We plan to use the ion channel "NAN-IAV mode" to construct auditory mechanical transduction in drosophila, introduce the saccharomyces cerevisiae experiment, establish an auditory module to study this life phenomenon, and do the first round of research on cancer perception. The acoustic transduction of microorganisms was discussed. Our project will focus on the expression of NAN-IAV ion channels in Saccharomyces cerevisiae, and amplify the cytoskeleton using line (Suntag scFv) of downstream signals, so as to realize mechanical stimulation of physiological activities, observe the response of yeast cells to sound waves, changes in gene level, protein level, metabolism and growth phenotype.
Our experiment is only the initial contribution to cancer cognition, because cancer treatment and prevention is a very important and difficult process. We hope to pave the way for future teams. We hope that future technologies or new achievements can help us transform Saccharomyces cerevisiae and provide a new method to detect cancer.
The second reason why we chose to participate in this project is that we have some ideas about "animal hearing aids". Our research on cats shows that their ears have a high demand for "hearing aids" and other products, which reminds us of the research ideas of "sound perception" and "sensors". We began to focus on the direction of sound transmission and response reception. This has proved that our current project's illusion of cancer perception is also to transform Saccharomyces cerevisiae into a "sound sensing" link similar to a sensor. The cat hearing aid project that our team members participated in at the early stage really played an important role in determining the direction of the project.
Based on the above reasons, we finally chose to continue this research and develop it as a cohesive and meaningful technical means, and named it "Put your ear on the yeast to hear the voice of the world".

Cancer perception






We intend to use the "Nan IAV mode" of the ion channel that forms drosophila's auditory mechanical transduction to introduce Saccharomyces cerevisiae to build an auditory module to study this biological phenomenon as the first round of the cancer perception project. The follow-up study of sound transduction in microorganisms was preliminarily discussed. So that more patients can get more timely treatment at the early stage, and improve the treatment efficiency.
Our products are more stable than the existing electrical equipment. Electrical equipment cannot work without electricity. If we have a power failure, the equipment will fail. Our products are stable, we do not need electricity or other things, and theoretically can work for a long time.
In real life, we will use the principle that cells can make sounds, and the feature that transgenic bacteria can respond to the sounds made by cancer cells, and apply it to the instruments that detect the location of cancer cells to find cancer cells and provide help for cancer treatment.


Seismic survey




We plan to use this "bacteria that can sense sound" in seismic survey.
Seismic wave is the spread of mechanical motion, which is generated by the elasticity of the earth medium. Its property is very close to sound wave, so it is also called ground sound wave. Earthquakes do great harm to human nature, and the self rescue time is short after the occurrence, so it is particularly important to predict earthquakes and take corresponding measures. We consider to connect this bacterium to the ground, observe seismic waves and monitor crustal activity with the help of the ground display through the transmission of electrical signals, which may be able to predict earthquake disasters and reduce the risk of erroneous measurement. At the same time, the cost of existing seismic monitors is relatively high, and the development of bacteria survey means can effectively reduce the cost.