Our project is devoted to the research and deepening of sound, which comes from a report on "cell acoustics" we saw at the beginning of the project. If all cells do beat like heart cells, is the vibration enough to produce detectable sound? Can we make our bacteria sense this sound? Is such research useful? We are looking forward to following the clues of cellular acoustics. Fortunately, we found this information from the data: "each cell produces its own unique sound." "Motor proteins are connected to the cytoskeleton (responsible for maintaining the structural integrity and shape of cells), and the cytoskeleton is connected to the cell membrane. Therefore, the movement of motor proteins is thought to be transmitted to the cell membrane through the cytoskeleton, leading to cell membrane oscillation." "The unified movement of intracellular motor proteins is considered to be the reason behind the cell sound." These data support our idea to a certain extent. As we all know, the most interesting application in the field of cell acoustics is the detection of cancer and other diseases. We envision that the presence of cancer cells can be tested by using the difference in cell vocalization or the perception of this special sound. So the first development direction of our project is to make cells use sound to sense cancer through operation. Hearing is the perceptive ability of animals to sound, which is the process of converting mechanical energy into electromagnetic signals. However, the mechanism is not completely clear, and whether there is a similar sensory system in microorganisms has not been studied. BJWZ-China is ready to try to create a precedent. We intend to use the ion channel "NAN-IAV mode" in Drosophila, which constitutes the auditory mechanotransduction, to introduce it into Saccharomyces cerevisiae in the experiment, to build an auditory module to study this life phenomenon, and to do the first round of research for the project of cancer perception. The following research on sound transduction in microorganisms was preliminarily explored. Our project will focus on the expression of NAN-IAV ion channels in Saccharomyces cerevisiae, and amplify the downstream signals to the cytoskeleton by using Tether (Sunag scFv), so as to realize the transformation from mechanical stimulation to physiological activity, observe the response of yeast cells to acoustic waves, and the changes in gene level, protein level, metabolism and growth phenotype. Our experiment is only the initial contribution to the perception of cancer, because the treatment and prevention of cancer is a very important and difficult process. We hope to pave the way for the future team. We hope that future technologies or new achievements can assist our transformed Saccharomyces cerevisiae and provide a new way to detect cancer. The second reason why we chose to engage in this project is that we have some ideas about "animal hearing aids". Our research on cats shows that their ears have a high demand for products such as "hearing aids", which reminds us of the research ideas of "sound perception" and "sensors". We began to focus on the direction of sound transmission and response acceptance. It has been proved that our current project's reverie of cancer perception is also to transform Saccharomyces cerevisiae into a "sound sensing" link similar to sensors. The cat hearing aid project that our team members associate with in the early stage has indeed played an important role in determining the direction of the project. Based on the above reasons, we finally chose to continue this research and develop it as a cohesive and meaningful technical means, and named it ---- "putting ears on yeast, hearing the voice of the world".