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An Huang Oct 3, 2022

Experiments

We conducted multiple experiments to test our design. This is a collection of all protocols we used for our experiments.

Protocols employed in experiments

Protocol name URL
Agarose gel electrophoresis https://www.protocols.io/view/dna-gel-electrophoresis-q26g74j61gwz/v1
Colony PCR https://www.protocols.io/view/colony-pcr-bp2l69bnklqe/v1
Determining biofilm growth amount (absorbance) https://www.protocols.io/view/determining-biofilm-growth-amount-absorbance-n2bvj8x95gk5/v2
Determining biofilm growth amount (dry weight) https://www.protocols.io/view/determining-biofilm-growth-amount-dry-weight-5jyl8jny9g2w/v1
E. coli growth curve measurement (culture with silver ion) https://www.protocols.io/view/e-coli-growth-trend-measurement-culture-with-silve-81wgby1byvpk/v1
Microwave digestion for microbes and inductively coupled plasma mass spectrometry https://www.protocols.io/view/microwave-digestion-for-microbes-4r3l2747xg1y/v1

https://www.protocols.io/view/inductively-coupled-plasma-mass-spectrometry-icp-m-x54v9dj2pg3e/v1
Obtain LB broth agar plates (with antibiotics) https://www.protocols.io/view/obtain-lb-agar-plates-with-antibiotics-j8nlkw4b5l5r/v1
Obtain LB broth medium https://www.protocols.io/view/obtain-lb-broth-medium-5qpvoryxdv4o/v1
Plasmid transduction using competent cell https://www.protocols.io/view/plasmid-transduction-using-competent-cell-j8nlkw4e5l5r/v2
Real-time quantitative polymerase chain reaction (RT-qPCR) https://www.protocols.io/view/real-time-quantitative-polymerase-chain-reaction-r-j8nlkkqmxl5r/v1
RNA extraction from E. coli https://www.protocols.io/view/rna-extraction-from-e-coli-j8nlkw44wl5r/v1
Sample preconditioning before scanning electron microscopy (SEM) https://www.protocols.io/view/sample-preconditioning-before-scanning-electron-mi-36wgqj465vk5/v1
Transform pCas9 https://www.protocols.io/view/transform-pcas9-eq2lynbopvx9/v1