Thanks for your attributions!

We would like to express deep and sincere gratitude to all the people, who helped us in competition. Their cooperation and encouragement keep us improving our project.

Roles of team members

General support

This indicates the various aspects of help to our overall project, such as team organization, the financial support, question consultation and so on.

Dr Guoxia Han:

Our primary PI, gave us financial support, experiment design and time planning suggestions. He controlled the quality of our work and inspired us go ahead.

Dr Dechang Xu:

Our second PI, gave us advice on modelling improvement, results analysis, and academic writing skills.

Dr Zheng Chen:

Expert in environmental chemist and pollutants transformation. Gave us support by listening all our concerns. Furthermore, he offered valuable help at every stage of the project, sharing new ideas for project improvement.

Ziwen Xie, Xinyan Han, Xinchen Nie, Yiwei Yang, Lizhe Chen, Shuhuai Yang, Simiao Wang, Weilin Liu:

Supported in team organization and encouraged us when we meet problems. They participated in our team building and accompanied us all along.

Department:

We would like to express greatest thanks to the department of Biological Sciences, Wisdom Lake Academy of pharmacy and the Department of Health and Environmental Sciences for financial support, laboratory platform providing and experiment training.

Wet-Lab support

Wet-lab experiment this year was designed and conducted by Weizhuo Chen, Qiaowa Gong, Fengyi Guo and An Huang

People who helped us:

Dr Guoxia Han
Gave us advice on experiment design, results presentation, who also organized and managed wet-lab experiments.
Dr Ferdinand Kappes
Gave us advice on plasmid construction and protein folding. He proposed us to think about bio-safety seriously and recommended experts in the relevant field enthusiastically.
Dr Sekar Raju
Suggested on experiment design such as measurement on E.coli growth curve, biofilm formation. Furthermore, he shared some feasible solutions handling on bio-safety problems.
Dr Carlos Lafourcade
Strengthened our understanding on GS-linker and protein folding. Molecular biology he taught us are really beneficial for experiment.
Dr Sekar Raju
Suggested on experiment design such as measurement on E.coli growth curve, biofilm formation. Furthermore, he shared some feasible solutions handling on bio-safety problems.

Ziwen Xie
Provided a Day-to-day support for whole experiment process. Supervised and gave suggestions during experiment.
Xinyan Han and Xinchen Nie
Gave us advice on experimental procedure and experimental operation such as RT-PCR and gene knockout.
Xiaohan Zhang, Zixin Rong and Yuwei Han
Provided advice on protocol optimization, and clarified experimental logic chain with us.
Sha Zhang
Helped us a lot on measuring metal ions adsorption, shared instruments using and data processing methods with us.
Keran Jiao
Gave us advice on nanoparticle observation and SEM using.

Zhongkai Huang and Sijing Meng
Supervised instrument using and provided basic lab reagent. They spent huge efforts to guarantee our experiments develop successfully.
Xia Huang and Xiao Zhou
took charge for our lab access and safety training.
Yulan Yi, Manting Qi and Xiaoping Xie
Gave technical support on RT-PCR, microplate reader and ICP-MS, also provided some reagents to us.

Other support
Qiaoli Feng, Bingjie Jiang, Jiahao Cui, Yifan Jiang

Enterprise
Genewiz, Genescript for gene synthesis, Yuantest for scanning electron microscope (SEM)

Modeling support

Modeling this year was designed and conducted by Wen Li, Jiayi Li and Jiayi Liu

People who helped us:

Dr dechang Xu
Gave suggestions about the building of the models of Molecular modeling along with biological pathways, cell working limits and growth curve analysis and related assumptions.
Dr Pow Seng Yap
Gave suggestions on the simulation of the function of device and practical use.
Lizhe Chen
Gave some instructions on the engineering mindset and hands-on cellular kinetics simulation.
Lanwei Zhang
Give us some computational idea regarding protein rational design.

Human practice support

Human practice this year was designed and conducted by Qiayi Zha and Xingshen Li

People who helped us:

Yiwei Yang

Ms. Yang is a member of the 2021 iGEM XJTLU-CHINA. She taught us the idea of HP research and guided us to think about the relationship between HP and society. At the same time, she clarified the logical chain of integrated human practices for us from the perspective of past people, and modified the wiki's presentation problem.

Dr. Zheng Chen; Dr. Zhengyu Hu; Dr. Raju; Dr. Kappes; Dr. Pow-Seng Yap;

Hardware support

Hardware this year was designed and conducted by Baiding Chen

People who helped us:

Lizhe Chen:
Provided software installation packages, assist in drawing hardware engineering drawings, assist in designing hardware dimensions, runners, and shapes; in circuit construction projects, assist team members to optimize sensor programs.
Hao Liu:
Helped us with 3D printing of hardware, solve device sealing problems and provide assembly materials.
Shengkai Sun:
Assisted in purchasing hardware materials.

Webpage support

Webpage this year was designed and conducted by Baiding Chen and Qiayi Zha

People who helped us:

Simiao Wang:
Instructed the application of poster software, assist in web page layout and beautification, and provide experience in video production.
Shengkai Sun:
Made a campus network and upload iGEM information.

Important literature references

Sponsor

Genescript and Genewiz: Offered discount price for gene synthesis.
Snapgene: Sponsored 12 software licenses.
Monad: Provided gifts and drinks are provided at communication event.

Team training and Project start

During winter vacation, iGEM 2021 XJTLU-CHINA provided iGEM training for us. Training coursed were divided into five sections: 2021 XJTLU-CHINA project introduction, literature reading methods, experimental planning, modeling skills, and HP instroduction. We also watched previous iGEM XJTLU-CHINA training videos, which are freeely available online and we benefit a lot from them.

We started working on project in February, finding problems in life that could be solved with synthetic biology. We brainstormed and identified our project topic "Application of new biofilm in industrial wastewater treatment" in May. We then began to reserve laboratory, design experiments and purchased experimental reagents. We got laboratory access permission in June. After that, we completed laboratory safety training and practiced basic experiment operation under the guidance of technicians.