Overview
The first section of the part collection (BBa_K4271000 ~ BBa_K4271003, BBa_K4271015, BBa_K4271016) is designed for the phosphate elimination functions, the increase of hydrolysis and absorbance of organic phosphate, in bacterial cells. The parts in the second section of the collection (BBa_K4271004 ~ BBa_K4271011, BBa_K4271018, BBa_K4271019) are designed for the construction of biosensors that could help monitor the functions of the parts in the first section. This is a comprehensive collection that includes both the parts with functions and the parts that assist the measurement of the said functions. This combination not only provides methods for phosphate elimination in different fields of application but also allows future users to more easily replicate experiments that could monitor the effectiveness of their constructs designed with these parts. Our part collection also included the integration of ideas from our partnership with another iGEM team, particularly in the use of a degradation tag (BBa_M0052) to improve the experimental results of biosensors.
Parts for Eutrophic Water Purification (OPH and AsPhoU)
Basic Parts
Part Number Description Diagram
Organophosphate Hydrolase + his-tag (OPH)
Antisense-PhoU (AsPhoU)
pelB signal peptide
AsPhoU RBS
Composite Parts
Part Number Description Diagram
T7 Promoter + Lac operator + RBS + pelB + OPH + his-tag + T7 terminator
araBAD promoter + RBS + AsPhoU + T1 T2 terminator
Parts for Biosensors (pNP sensor & polyP sensor)
Basic Parts (pNP sensor)
Part Number Description Diagram
pNPmut1-1
pobA/R dual directional promoter
pNP RBS
pobR operator
Basic Parts (polyP sensor)
Part Number Description Diagram
Tac promoter
rpoD sigma factor
ureA promoter
Composite Parts
Part Number Description Diagram
pNP composite
BBa_K4271004
BBa_K4271005
BBa_K4271006
BBa_K4271007
PolyP sensor with SsrA degradation tag
BBa_K4271009
BBa_J61101
BBa_K4271010
BBa_M0052
BBa_B0015
BBa_K4271011
BBa_K4271012
BBa_J18932
BBa_B0015
PolyP sensor without SsrA degradation tag
BBa_K4271009
BBa_J61101
BBa_K4271010
BBa_B0015
BBa_K4271011
BBa_K4271012
BBa_J18932
BBa_B0015