This year, we were inspired by SZU-China 2017 team and plan to improve their part for carbonic anhydrase. We took the existing part BBa_K2232014 and plan to clone into our cumate inducible pCT5c plasmid, with codon optimized sequence. We have successfully cloned their original part into pCT5c plasmid and characterized by carbonic anhydrase assay and biocementation assay. Due to time constriants, we could not get our improved part into our plasmid. However, we still believe that our design was rigorous and could be transferred to other teams in the future.
| Part number | Type | Name | Short description | Link |
|---|---|---|---|---|
| Improved Parts | ||||
| BBa_K4417020 | basic | CA22 | Improved part from BBa_K2232014 with codon optimization | http://parts.igem.org/Part:BBa_K4417020 |
| BBa_K4417021 | composite | CA22- rrnB T1 Terminator | Improved composite part from BBa_K2232014 with codon optimization | http://parts.igem.org/Part:BBa_K4417021 |
| BBa_K4417022 | composite | CuO-RBS-CA22-rrnB T1 Terminator in pCT5c | Improved composite part in pCT5c Type IIS compatible plasmid | http://parts.igem.org/Part:BBa_K4417022 |
| BBa_K4417023 | composite | P43-RBS-CA17-rrnB T1 Terminator in pCT5c | BBa_K2232014 in pCT5c Type IIS compatible plasmid | http://parts.igem.org/Part:BBa_K4417023 |