The experimental workflow shown below is used when we design any of our genetic constructs. The protocols used in the process below are included on this page in addition to other protocols we used for our project.
We had to spend a significant portion of our time on Wet-Lab work this year optimizing a protoplast isolation protocol for wheat plants. This was a key step in being able to test our heat-inducible promoters in wheat cells. The process below summarizes the protoplast protocol.
Download Protoplast Isolation & Transfection Protocol