| Criteria |
Achievements |
Wiki Links |
| Competition Deliverables |
Our deliverables, including our Wiki, Project Promotion Video, Team Presentation, and Judging Form, have all been uploaded and included in our account or wiki. |
N/A |
| Attributions |
We value the work our teammates put into our project this year, and have listed every team member's contributions. |
https://2022.igem.wiki/ubc-vancouver/attributions |
| Project Description |
The description of our project opens up our story, leading into our design for wet-lab, dry-lab, and human practices aspects. |
https://2022.igem.wiki/ubc-vancouver/description |
| Contributions |
We created 3D-printed hardware and an Arduino-based fluorometer system, and included documentation to share knowledge with other teams on how to build such a model. |
https://2022.igem.wiki/ubc-vancouver/contribution
https://2022.igem.wiki/ubc-vancouver/model#hardware |
| Criteria |
Achievements |
Wiki Links |
| Engineering Success |
As a genetic engineering team, we iterate through the engineering design cycle at the scale of our experiments, our constructs, and our project as a whole. Using protoplasts as our proof of concept system proved the need for various design cycles to optimize our protocol and yield of our isolation for protoplast transfection. |
https://2022.igem.wiki/ubc-vancouver/engineering |
| Collaboration |
Throughout our project, we provided support and advice to the UBCO team in their first iGEM year, being from a different campus of our same university. This proved to be mutually beneficial since they obtained heavy mentorship and advice as first-timers that we had not received in our time, while we obtained resources and advice they got as a first-time team that we were unaware of. We met both virtually and in-person in a Vancouver visit to discuss our projects, methods to narrow down idea generation, and research storytelling, and we toured them around our lab space. As teams from sister campuses, we split a discounted registration fee, helping each other financially. Additionally, they organized the Phototroph Community meetups to foster a community of iGEM members working with phototrophs, where we exchanged information with others working on plant synthetic biology to ameliorate our protocols. It also contributed to our ability to aid future teams since we produced a detailed wheat protoplast isolation protocol as part of the Phototroph Community Handbook for future teams’ reference. Hence, this was a mutual exchange of useful information. |
https://2022.igem.wiki/ubc-vancouver/collaborations |
| Human Practices |
In creating a project that is meant to serve nearly all citizens of the world, we want to ensure that there is adequate need and acceptability of this technology throughout the various stakeholders of our project. Please navigate the Integrated Human Practices and Entrepreneurship work carried out during our project. |
https://2022.igem.wiki/ubc-vancouver/human-practices
https://2022.igem.wiki/ubc-vancouver/entrepreneurship |
| Proposed Implementation |
We need to consider the downstream work our project would require if scientific validation was successful. For this purpose, we consulted with various stakeholders and end-users about the best possible implementation of our project in real farms and how it would fall into consumer’s hands. |
https://2022.igem.wiki/ubc-vancouver/implementation |
| Criteria |
Achievements |
Wiki Links |
| Integrated Human Practices |
Given that our team had not worked with plant systems before, we went through thorough consultation to ensure our approach was valid, as well as the need for our project and genetic reasoning was sound. Our team decisions were based on these outcomes. All of our interviews with experts and stakeholders is in our Integrated Human Practices page. |
https://2022.igem.wiki/ubc-vancouver/human-practices |
| Models |
We complemented certain aspects of the wet-lab with dry-lab efforts. We confirmed that the pathways that our overexpressed genes would affect were differentially expressed in heat stress, and aimed to develop a hardware component that would measure the activity of our system in real plants. |
https://2022.igem.wiki/ubc-vancouver/model |
| Partnerships |
Throughout the duration of our project, we collaborated with the IISER Pune team for human practices, wet-lab, and dry-lab efforts, in the common theme of both our projects overexpressing the enzyme ACC deaminase. The connection between our teams during different points in the year was greatly beneficial to varying aspects of our project. |
https://2022.igem.wiki/ubc-vancouver/partnership |
| Education & Communication |
Our project aimed to ensure sustainable production systems and food security; however, for this, our consumers and end-users must be appropriately educated to receive this technology appropriately. |
https://2022.igem.wiki/ubc-vancouver/communication |
