Protocols

Materials

1. 50% Glucose Solution

2. 0.1M HEPES Buffer (pH 7.5)

3. Antibiotic Stock Prep

Media

1. BG11 Liquid Media

2. BG11 Agar Plates

3. LB Liquid Media

4. LB Agar Plates

5. Selection Media Agar Plates

Culturing

1. Liquid Media Inoculations

2. Mother Cultures

3. OD Measurements

Transformations

1. E. coli Heat Shock Transformation

2. Natural Transformation of Cyanobacteria

3. E. coli Electroporation

Cyrostocking

Cryostocking

DNA Extraction, Amplification, and Analysis

1. Monarch Plasmid DNA Miniprep Kit Protocol

2. Colony PCR Validation

3. Golden Gate Reactions

4. Analytical Digestions

Assays

SDS-Page