Notebook

8/14

1. We know each other and get familiar with each other quickly through ice-breaking games.
2. In order to let us to understand our project in depth, the wet team coach led the students to do a project review.
3. The probationary group captains were elected. we also created an official account on WeChat, designed the team name and Logo, and conceive a series of teams’ souvenir.

8/15

1. We learned the safety knowledge related to the laboratory and the matters needing attention in the experimental operation;
2. LB and YPD media were made.

8/16

We learned about the principle and application of PCR, after which each one performed the process of configuring the PCR system for ACC1, FAS1, and FAS2, followed by PCR reactions for these three enzymes and the process of running gel, and finally observed the experimental results.

Table 1: PCR Process

8/17

First, we use Gel DNA Extraction Kit to extract ACC1, FAS 1 and FAS2 genes that were got yesterday. Then we divided into three groups to transform ACC1, FAS1 and FAS2 respectively. After that the connected target genes were transformed into Escherichia coli, thus completing the plasmid construction.

Table 2: Gel DNA Extraction Kit component

8/18

We studied the coating plate using the plasmid that had been transformed yesterday and did the yeast inoculation experiment.

8/19

We checked if the PCR transformation products from yesterday grew on the solid culture plates, after which they performed colony PCR, use Gel DNA Extraction Kit to extract genes, and placed into the media overnight for incubation. At the same time, the preliminary preparation of yeast competent cell was carried out.

8/20

Preparation for the yeast competent cell.

Preparation for the yeast competent cell.

At last, we transform the plasmid that we extracted to the yeast competent cell.

8/22-26

Selection of monoclonal strains, add then into YPD media to do the fermentation cultivation. After that, using microplate reader to get the growing curve of fermentation strain. The fermentation samples were sent to test the content of ethyl hexanoate

Table 3: the value on the microplate reader

8/27

Individual and team photo shooting

Assigning the task of writing wikis and parts

8/28-9.12

Write wikis and parts; Prepare the Powerpoint for pre-presentation.