Team members
Adam
Johansson
Holopainen
Adam is our design guy! He managed the production of our promotional video together with Sumant and Daniela, and was responsible for creating the cool assets and several of the animations in our videos, as well as illustrations on our team clothing and social media posts. Outside of his expertise with the drawing pad, he spent a lot of the summer participating in the wetlab effort, being involved with minipreps, colony PCR, and a few of our successful clonings.
Aishwarya
Sajimon
Aishwarya was part of both the drylab and wetlab endeavours throughout and acted as a bridge between both when needed. Being a valuable member of wetlab, throughout summer, she picked up various tasks in the bacterial lab and actively participated in cloning and site-directed mutagenesis experiments (SDMs). She also contributed to primer design and optimization of PCR protocols for SDMs during the troubleshooting phases. As part of drylab she was involved in the various in-silico modelling efforts
Amela
Peco
Amela has carried our human practices to success: she has written a children’s book, prepared classroom activities and interviewed many experts throughout the project. She has also been adopted into the wetlab crew during the summer and she became a workhorse creating mutants and midi-prepping during her free time. With her young age, we don’t expect anything else from her other than becoming a great iGEM leader in the future.
Barathram
Swaminathan
Barathram is our lab guru. He’s been involved in pretty much every step from the project conceptualization and development to the validation studies at the end, with every step in between. His knowledge, experience and quick skills in wet lab were crucial during our troubleshooting phases, which would have ended up a lot more painful without Barath.
Daniela
Weh
Daniela was our event manager for SiC, the wetlab taskmaster and a media person. She took up the monumental responsibility of the mutant production part in our project in its entirety and pulled it off. She co-ordinated the “big team” to produce the mutants at breakneck speeds and her long portfolio of other iGEM obligations. For the media team, she contributed with texts, postings and especially with directing the promotion video production alongside Adam and Sumant. Together with Pragha and Sumant, she came up with the idea of organising the first Swedish iGEM conference - short “SIC” - and created the foundation on which subsequent iGEM teams can build up a new tradition.
Evgenia
Paraskevi
Ntaouka
Or Zenia, has been instrumental in bringing several ideas of our wet lab to fruition. She has been involved in helping with the cell culture part of our project apart from offering her never-ending but crucial observations and opinions which shaped the project. She has been a part of other wetlab tasks such as cloning, creating mutants and prepping for the steps in between. In addition, she got involved in media work with promoting our project to the general public through our social media accounts.
Joël
Tekoniemi
Joël is one of the two team leaders with a focus on leading wet lab and human practices as well as co-leading the finance subteam. He has an eye for optimization - relentlessly searching for ways to optimise the experiments and to extract every ounce out of them. He was involved in planning and executing some of our experiments, ordering materials and reaching out to sponsors. He also performed interviews and went to school visits and headed our partnership with UZurich.
Karolina
Gustavsson
Karolina was part of Drylab and has mainly focused on immunogenicity modelling using both linear and discontinuous epitopes. Besides the immunogenicity modelling she also did a bunch of smaller things like modelling thermodynamics between IF and cubilin with Mutabind2.
Keerthi
Anand
Raghavender
Keerthi is another cornerstone of wetlab, who was indispensable with her experience in cell culture part of wet lab. Apart from leading the team for setting up the transfection, western blotting, flow cytometry and fluorescence microscopy experiments, she solely led the cell culture faction for the entirety of its execution. She managed to reiterate the protocols until she and the instructors were satisfied. With parallel tasking master thesis and iGEM, she really made bought the vision of the project (Antibodies binding and B12 binding) successful in all her attempts.
Lumeng
Li
As one of the two team leaders, Lumeng mainly led the media and drylab teams as well as co-leads the finance team. Her involvement in the successful grant application was a solid foundation for the smooth progress of the team's project at KI. In addition to funding, she has been instrumental in getting the KI lab up and running. Her good communication with the PI, instructor, advisors, doctors, investors, and sponsors was key to our project's continued improvement with external voices. In research, she has worked on the application of fluorescence detection (also conducted Interlab using plate reader), specialised in image analysis and participated in the development of HEK cell experiments using fluorescence microscopy.
Malcolm
Halvarson
Malcolm was primarily a part of the wetlab team, helping to produce the mutants by setting up PCRs, making cultures and running endless minipreps. In addition, he helped develop ideas in the HP team.
Mariia
Minaeva
With her expertise in Bioinformatics, Mariia has been the pillar of the dry lab team. Not only has she created the script to generate our mutant proteins, she also successfully designed all the primers needed for the site-directed mutagenesis. As co-creator of the ProMutor software, she also mastered the proteins structures prediction, the docking study, and actively participated in every media task.
Praghadhesh
Thiagarajan
Pragha has been the solid link between the Wetlab and Finance team, but focusing a lot on both. Having a firm hand on the ever-increasing expenditures of the wet lab team, he was pivotal in navigating the corporate trenches getting us better deals at every turn. Thanks to him we have obtained grants and sponsors and he has made a substantial amount of mutants and cultures as well. You can often find him typing emails to prospective companies, drafting proposals and as well as organising fikas.
Raphaël
Mauron
Raphaël is our wiki manager, coder, designer,.. Well, without him the wiki would not have existed. Raphaël was part of both wet lab and dry lab team until the wiki marathon began. Despite supporting the wet lab work with his expertise in designing plasmids and inspiring the whole team with his amazing project proposal, he successfully used JavaScript to bring to life our ProMutor software. He also mastered web design and development for both our first official iGEM Stockholm webpage and Wikipage. You won’t believe it when we tell you that he is doing these things for the first time!
Shuanghao
Tang
Primarily a finance team member who has been the point of contact with banks in order to sort out internal fund transfers. Apart from that, she is involved in budgeting, finding external collaborations and sponsors, as well as pitching the product to different incubators. She has assisted the team with various grant applications, which served as a primary source of funding for the project.
Sumant
Salphale
A primary media guy, Sumant managed all of our social media channels and contributed to their content with stunning views from his drone! By listening to so many native speakers in different languages while recording and editing the 11 voice overs of our video, he must be a language expert by now. His ability of fast-understanding made him an amazing mate in the lab. His acumen and sincerity shines through even in wet lab experiments where he was responsible for getting us that perfect cloning result. He was the guy everyone called when the autoclave gave us problems. He also got dragged into almedalen and to perform interviews which earned him a role as honorary member of human practices.
Tingting
Zhou
Tingting Zhou is a member of the finance team and human practices team. As a finance team member, she has co-ordinated with KI Board and KI Innovation for grants. Besides, she contacted some early-stage Venture Capitals for advices of potential business model. Being a part of the human practices team, she is always thrilling to get more feedback about our project from society. For this, she interviewed doctors and consulting companies to understand the current incidence, risks, diagnosis and treatment of PA in clinical trial, and checked the regulation of the application of drugs and food.
Principal Investigators
Prof. Johan Rockberg
Professor in antibody technology and directed evolution at the department of Protein Technology, KTH
Johan is our primary PI and he has always been there for us, for administrative questions most of the time, but also with crucial guidance and advice that not only helped us but shaped the project. His expertise in epitope mapping inspired our project to tackle antibody binding, with a twist. We are incredibly thankful for Johan for having allowed us to take part in this journey, and we hope to have made him proud of us.
Prof. Ute Römling
Secondary iGEM PI and the head of a research group at the Department of Microbiology, Tumor and Cell Biology at Karolinska Institutet with a research focus on microbiology. She helped us by providing timely suggestions and advice, DH-alpha cells for inter-lab, and contacting a working space in IMM for level 2 cell lab.
Instructors
Aman Mebrahtu
PhD student
Aman was the most inspirational instructor we had. He asked us the right questions at the right time. He ensured that we understood each part of the project and gave us a clear idea of what to expect from each project segment. He helped us with our cloning strategy by giving us an introduction to SnapGene and also sharing his experience so that we were able to avoid time-costing mistakes. He always gave alternate suggestions to our problem-solution even during his busy thesis days. He was equally invested in the success of our project as we were.
Marit Möller
PhD student
Marit has helped us shape our project since the beginning, with valuable advice and helping us place orders quickly. She specializes in the field of Protein engineering and contributed to the project by imparting knowledge at key moments.
Magnus Olsson
Senior research specialist in Molecular Toxicology at Karolinska Institutet, Institute of Environmental Medicine, Unit of Systems Toxicology. Apart from providing us with access to the level 2 cell lab and the cells for our experiments, his advice and guidance were instrumental in several stages of our planning and execution. Being an excellent supervisor, he was extremely patient, receptive to all our questions, and encouraged us to explore new strategies and techniques.
Advisors
We were fortunate to have a large team of eager researchers around us in the lab, who were or became our advisors during the project. We always knew that someone with the special skills and knowledge for a specific issue would be there and happy to help us, which made our lives a lot more comfortable while working in their lab.
Hannes Thorell
PhD Student
Hannes was there from the beginning when we were discussing our project during our brainstorming phase and when we were developing our project. He enabled us to get access to a cell lab and allowed us to obtain our transfection results.
.
Malin Jönsson
PhD student
Malin works with protein engineering in relation to developing novel therapy approaches. She guided the team in the project planning phase.
Maximillian Karlander
PhD student
Max is working with gene therapy and affinity protein engineering. He guided the team with variant design, cloning and selection of antibodies based on the applications.
Num Vistbacka
Laboratory engineer
Num has been instrumental in guiding us through the entirety of western blot. He was easy to approach in the lab whenever we had doubts, even in between his experiments. He was always ready to troubleshoot and share his knowledge of bacteria as well in cell culture experiments. He always gave us the confidence to perform complex protocols by hand-holding us in each step.
Emma Vincent
Senior researcher in Molecular Toxicology at Karolinska Institutet, Institute of Environmental Medicine, Unit of Systems Toxicology. As a financial supervisor, she helped us with grant applications at KI. As a scientific advisor, she helped us order lab materials for our experiments in IMM.
David Hörl
Postdoc at Department of Human Biology & Bioimaging at Ludwig Maximilian University of Munich. He gave us a lot of insights into fluorescent microscopy by walking us through all the practical and technical details in terms of the difficulties and beauties of image analysis based on his experience and expertise.
Experts
Frédéric Mauron
Engineer in Microelectronics
Frédéric has been of the greatest help on the technical parts of the software creation. With his availability, knowledge and faith in our side project, he successfully guided us in the darker areas of software creation. By having multiple looks at our bugs and providing priceless advices, we successfully glued the pieces together. Last but not least, we are remarkably thankful for the new server he put at our disposal for free.
Alexey Sizykh
As an expert in software development, Alexey played a crucial role in bringing our software to life. With his knowledge in web and software development, he helped us resolve all the struggles with connecting frontend part of our software to its backend. Without his help and support, it would have been impossible for us to make the magic happen.
Sponsors
Royal Institute of Technology served as the primary sponsor of iGEM Stockholm 2022. As Sweden's largest technical research and learning institution, it’s a centre of talent and innovation. KTH provided us with CBH funding, lab space, equipment and materials for our bacterial and HEK 293T experiments.
Karolinska Institute was the second major sponsor of iGEM Stockholm 2022. KI is one of the world's leading medical universities. This year, KI supported us financially with two grants (KI Board grant and ToxiMaster grant), a level-two cell lab for HEK 293T experiments in IMM with equipment and basic lab materials.
An incubator, located inside of Karolinska Institutet Solna campus. It supports research groups in life sciences to develop business model for their projects evaluated with commercial potential. KI innovation not only provided us with a grant but also bridged us with investors and consultants, and gave us several workshops.
iGEM is a synthetic biology competition which has motivated students from various backgrounds to learn about synthetic biology. iGEM has supported 90 teams with the most impactful projects annually by providing grants and incentives. We thank iGEM for the support and trust they placed in our impactful project.
A company that is dedicated to providing the scientific community with high-quality recombinant proteins and antibody reagents to promote life science research and drug discovery. As our biggest sponsor of antibodies, this company provided us with primary and secondary antibodies for different applications.
Atlas Antibodies is a Swedish company that has tremendous success in producing specific monoclonal and polyclonal antibodies toward proteins found in the human body. We thank them for the support they extended to us in the form of discounts while purchasing their primary antibodies.
Integrated DNA Technologies (IDT) company develops, manufactures, and markets nucleic acid (DNA) products that support the life sciences industry. IDT’s support has proven crucial for our project and we would like to thank them for providing us with gBlocks and primers.
A company that is deeply rooted in the fields of genomics, protein analysis, drug discovery and molecular biology. The Nordic division of the company was one of the earliest sponsors and has supported us generously by providing midi- and miniprep kits.
Microsynth is a leading European company in nucleic acid synthesis and analysis with three main business areas, DNA/RNA Synthesis, DNA/RNA Analysis and Sequencing, and Contract Research/Outsourcing. We are grateful for having received sequencing labels from them.
Bio-Rad manufactures genomics, proteomics, cell biology research, and clinical diagnostics products for medical, industrial, and academic research. Biorad supported us by offering advice and discounts on western blotting products and reagents.
BioRender is the world's first tool used to create, edit, and collaborate on diagrams, and illustrations, aiming to help scientists for professional scientific figures. Biorender provided us with a premium account, which we used throughout the project to communicate our solution in a clean, professional manner.
SnapGene is a user-friendly cloning tool which provides the easiest and most secure way to plan, visualize, and document molecular biology procedures for scientists. Snapgene provided 16 team members with a software license, allowing everyone to perform in silico cloning.
Beckman Coulter offers clinical diagnostic products and comprehensive clinical laboratory solutions backed by more than 80 years of experience and innovation. It operates in two industries: Diagnostics and Life Sciences. We thank Beckman Coulter for sending us their CosMCPrep kit for plasmid isolation.
New England Biolabs is one of the leading companies in the development of reagents for molecular biology. NEB has supported many iGEM teams by providing support kits for performing recombination. We thank New England Biolabs for providing restriction enzymes, ligases, and gel electrophoresis kits.