Team ice-breaking
Project Introduction
Project discussion
Laboratory Safety Training
Preparation of LB medium
PCR amplify parts of pUC57 and DexA70
Gel extraction
Homologous recombination
Transformation of plasmid into DN5α
Sending for sequencing
Coat plate for selection of plasmid
Plasmid Extraction
PCR verification of plasmid
Culture bacteria colony
Take team casual photo
Use CaCl2 solution for the preparation of sensitized cells
Repeat PCR
Preparation of LB medium
Determine the experimental scheme
Transform DexA and Cly R to E. coli Nissle 1917
Store samples of 96 well plate
Determination of redusing sugar using DNS method
SDS PAGE electrophoresis
Examine ability of film suppression and film removal
Repeat Transformation
Prepare His Buffer for the purification of protein
Repeat the storage of samples in 96 well plate
Repeat the DNS method
Repeat SDS PAGE electrophoresis
Preparation of Brain Heart Infusion Broth (BHI) medium
Culture Streptococcus mutans
Inhibition zone experiment of Streptococcus mutans
Collect and analyze experimental data and write wiki materials
