Caries is a bacterial infection that occurs in dental tissue. This project upgrades genetically engineered bacteria, which can not only specifically kill Streptococcus mutans that cause caries, but also effectively prevent Formation of biofilm and dissolution of biofilm that has already formed, preventing caries. The principle of this project is by constructing the DexA plasmid and the ClyR plasmid, using Escherichia coli Nissle 1917 strain to obtain DexA and ClyR engineering strains, and measuring the enzyme secretion and enzyme activity by SDS-PAGE and reducing sugar method, and testing its ability to inhibit the formation of Streptococcus mutans biofilm and break the biofilm achieves the dual effect of removing the biofilm barrier and lysing caries bacteria. This project has great potential in the prevention and treatment of dental caries.
