• The background, history, and scale of iGEM.
• Rules and where to find more information
• The products of some past gold-medalist teams
• A proposed timeline of our team’s progress

• Eukaryotes, prokaryotes, their differences and similarities
• Basic information about DNA, RNA, and proteins
• The central dogma
• Basics of genetic engineering
• What GMOs are, and their benefits

• Definition and features of market research.
• Macroµ market research
• Questionnaire design
• Analysis of collected data

• cloning and restriction enzymes.
• History of DNA structure, restriction enzymes, cloning, and Genentech.
• Watson and Crick’s discovery of the structure of DNA.
• Plasmids and some fundamentals of molecular biology.

• Tips & preparation for expert interviews.
• What to do during an interview.
• How to check information after an interview.

• Basic lab techniques
• Culture methods and procedures
• Structure and features of plasmids
• Protein expression and purification

• Activity planning and publicity for popular science education
• How to prepare materials for the iGEM competition

• What genes are and how they work
• More basic information about genes
• Biobricks and how to design or assemble them.

• Business planning and strategies
• How to improve our products from a financial perspective
• agenda, commercialization, implementation and entrepreneurship

• Explanation of modeling method and diagram drawing
• Components of a team for iGEM
• Requirements for awards

• Learn about laboratory safety and how to use mechanical pipettes
• Prepare LB liquid and solid culture media
• Lean the principles of molecular cloning
• Use PCR to amplify PKC gene sequence

• Use restriction enzymes to isolate target gene sequences from their original vectors
• Use gel electrophoresis to isolate gene sequences of the correct length
• Retrieve genetic material from the gel

• Transform TOP10 competent cells with pET-28a, XynA, PKC, and CcxynA.
• Amplify density of pET-28a, XynA, PKC, and CcxynA

• Learn about plasmid extraction
• Extract plasmids from TOP10 competent cells
• Pick monoclonal colonies of bacteria and inoculate in liquid medium
• Prepare liquid LB culture medium for further experiments

• Expand the culture of bacteria
• Test density of bacterial culture (detect OD value)
• add IPTG to induce production of target proteins
• Prepare buffers for extraction of proteins

• Extract and purify proteins from BL21 cells using ultrasonic lysis and Ni-NTA beads
• Test density of proteins in byproduct liquids to ensure all proteins have been extracted

• Prepare gel for SDS-PAGE (including Coomassie brilliant blue staining solution)
• SDS-PAGE
• Observe results

• Determination of the concentration of reducing sugar xylose with DNS and obtaining the standard curve
• Determination of enzymatic activity of four enzyme solutions ( xynA, PKC ,xyl3A and ccxynA )

• Determination of enzymatic activity ( xynA, PKC ,xyl3A and ccxynA )
• Experimental data analysis
• Writing experimental results