Pasture feed mainly includes silage and grain feed. Silage contains a large amount of lignocellulose, which is difficult to digest and absorb, while grain feed is composed of a large amount of hemicellulose xylan, which is not easily absorbed by animals. This project plans to adopt the multi-enzyme synergistic degradation strategy to construct engineering bacteria that can hydrolyze lignocellulose and hemicellulose xylan at the same time. Firstly, the recombinant plasmids of cellulase PKC and xylanase xynA were constructed and transformed into P. pastoris, and the positive transformants were screened to obtain the recombinant bacteria group with high enzyme production, and the enzyme activity and extracellular protein secretion were measured.This project can improve the utilization rate of silage and grain feed and their applicable breeds of livestock, and improve the economic value of these feed.