October
Team:
-Our team was officially formed, initially consisting of wet-lab group, dry-lab group and HP group.
-All members began to learn the excellent projects of the previous iGEM teams.
Brainstorm:
-Our team started the first round of brainstorming, in which the themes include the production of natural sugar substitutes, the biodegradation of metformin in the environment, high-precision neural/vascular binding, and the biosynthesis of atropine.
Brainstorm:
-We determined the research direction of our project, that is, the synthesis and application of terpenoids, and began to investigate and select terpenoids for microbial synthesis, including geraniol, limonene, patchoulol, medroxone, squalene, astaxanthin, lycopene, etc.
-At the end of November (11.29), we basically determined the framework of the project, that is, to biosynthesize lycopene and patchoulol using Pichia pastoris as the chassis, and apply them to silk as terpenoid dyes.
Team:
-The team regularly held brainstorming sharing every week. The theme included the characteristics of silk and its industrial development status, and the research progress of lycopene and patchoulol biosynthesis.
Wet-lab:
-All the iGEMers from our team learned laboratory fundamental qualities and received experimental skills training in molecular biology and microbiology.
-We carried out the lycopene dyeing experiment on silk, observed the dyeing effect and improved the dyeing conditions.
HP:
-We started to operate a Wechat official account dedicated to SUCT-China 2022.
Team:
-We recruited students from South China University of Technology with design, modeling or web building skills, and the new teammates became one of the members of the art group or web/modeling group.
Education:
-From 1.15 to 1.16, we held winter vacation biotechnology skill training for the School of Bioscience and Engineering of SCUT.
-We released a series of popular science push on WeChat official account, with the theme of wet experimental teaching of molecular biology and popular science of synthetic biology.
Wet-lab:
-We conducted a preliminary experiment on PTS mutation
-BBa_K4263007 was amplified from the genome of Pichia pastoris GS115.
-BBa_K4263008 was amplified from the plasmid available in our laboratory.
Team:
-The members of the art team completed the design of team elements, such as team logo, team uniform and mascot of FUNDYES.
Education:
-A questionnaire on the social acceptance of FUNCDYES was released.
-We started planning the "Run Plasmid" orienteering activity.
Education:
-On 3.13, we carried out the synthetic biology popularization activity, "Running Plasmid".
Wet-lab:
-On 3.17, BBa_K4263002 was amplified from the genome of P.pastoris GS115.
-On 3.20, successful construction of plasmid pPICZA-PPET9-EGFP-His.
-On 3.25, BBa_K4263000, BBa_K4263003, BBa_K4263004 and BBa_K4263006 were extracted from the genome of P.pastoris GS115 and amplified.
Team:
-After communicating with Prof Jianwen Ye on project design, the team decided not to limit to single static regulation, but to start the design of dynamic regulation.
Wet-lab:
-On 4.2, successful construction of plasmid pPICZA-P0547-EGFP-His, pPICZA-PDAS2-EGFP-His, pPICZA-PPGK1-EGFP-His and pPICZA-PGAP-EGFP-His.
-On 4.8, we linearized the above plasmids and electrotransform them into P.pastoris GS115 strain.
-On 4.22, BBa_K4263001 and BBa_K4263005 were amplified from the genome of P.pastoris GS115.
-From 4.25 to 4.30, shaker fermentation for 120 hours, and use the microplate reader to characterize the fluorescence of the promoter.
Brainstorm:
-Based on the photosensitive protein EL222, we hope to introduce the optogenetic system to improve our regulation means, that is, dynamic regulation.
Team:
-Our team was formally accepted by iGEM
Wet-lab:
-The first round of fermentation of engineering yeast containing patchoulol and lycopene synthesis pathway was carried out, and two terpenoids were analyzed by GC and HPLC.
Education:
-We finished the design of “Puzzle games!” with "Mr. yeast's adventure" as the background, and used vivid language to tell the public the rules of the game.You can play our game in our Wiki.
Team:
-We participated in this year's Mentorship Program on May 1st.
-We completed the design of the gene circuit of the optogenetic system in P.pastoris strain.
HP:
-On 6.3, our team attended the 6th Southern China Regional meeting. During the meeting, we established contact with SUSTech_Shenzhen.
-Exchange meeting with SUSTech_Shenzhen on 6.3.
Team:
-On 6.24, we submitted preliminary project safety form.
Wet-lab:
-We carried out the manganese ion concentration exploration experiment of PTS mutation.
-On 7.10, we submitted the sequence of BBa_K4263009 and BBa_K4263010 to Tsingke Biotechnology Co., Ltd. for DNA synthesis.
-On 7.19, successful construction of plasmid pPICZA-PACC1-EGFP-His and pPICZA-PERG9-EGFP-His, and we linearized the above plasmids and electrotransform them into P.pastoris GS115 strain.
-On 7.21, successful construction of plasmid pPICZA-PACC1-EGFP-His, pPICZA-PERG9-EGFP-His, pPICZA-PGAP-EGFP-His, pPICZA-PDAS2-EGFP-His, pPICZA-PPGK1-EGFP-His, pPICZA-P0547-EGFP-His, pPICZA-PPET9-EGFP-His and pPICZA-PAOX1-EGFP-His.
-From 7.24 to 7.28, we carried out the second round of fermentation of engineering yeast with EGFP as the reporter gene.
HP:
-Exchange meeting with GYHS team from Guangya Middle School on 7.15.
-Interview for SCUT-China 2023 on 7.20.
-Exchange meeting with NPU-China on 7.21.
Wet-lab:
-From 8.04 to 8.08, we carried out the third round of fermentation of engineering yeast with EGFP as the reporter gene, and obtained the data of promoter strength by analyzing the relative fluorescence unit.
-From 8.10 to 8.14, the second round of fermentation of engineering yeast 2OZPP containing patchoulol and lycopene synthesis pathway was carried out, and two terpenoids were analyzed by GC and HPLC. We obtained the production and titer of patchoulol and lycopene of engineering yeast corresponding to different promoters.
-Color complementary analysis of engineering yeast on 8.15.
HP:
-On 8.19, our team attended the Conference of China iGEMer Community.
-Exchange meeting with BiocreAtech (Shenzhen) on 8.23.
-Exchange meeting with USTC-China on 8.9.
Education:
-On 8.30, we went to Kangyuan Therapy Station with LZU-China for volunteer activities to help the disabled.
Wet-lab:
-On 9.9, we successfully constructed pPICZA-(C120)5-PminCYC-PTS-PGAP-TF-His.
-Replaced the upstream promoter of PTS mutation, and determined the appropriate manganese ion concentration. Carry out color complementary analysis and preliminarily screen PTS mutants.
-From 9.14 to 9.15, the last round of fermentation of engineering yeast 2OZPP containing optogenetic system was carried out, and two terpenoids were analyzed by GC and HPLC. In this round of fermentation, we obtained data to verify the blue light induced activation.
Education:
-Science Popularization Activity of Synthetic Biology in Guangzhou Guangya Middle School on 9.16.
HP:
-Meeting with NFLS_Nanjing & Nanjing-China on 9.30.