Proposed Implementation

1. What is our Target User Group:

From beginning to end, our main user group will be people who are overweight and people who eager to maintain a healthy body weight. We have made some interviews with doctors and made fairly good progress. The doctor has recommended that those who have BMIs that larger than should take out product. Moreover, we have done an extensive market basket survey, obtaining public acceptance toward weight-reducing drugs. In these regards, the main users in application scenarios will be those who suffer from complications of obesity or weight anxiety.

2. How to Produce Our Product?

We decided to use fermentation to obtain our functional strain. During fermentation, probiotics are produced in individual bioreactors and supplemented with specific prebiotics and carrier agents to enhance performance. Then, the cultured E. coli will be freeze-dried to a solid form to achieve an acceptable shelf. (Fenster et al., 2019). We are stunned by the technical maturity of this method——to reliably and reproducibly produce high-quality, safe, and stable probiotics and what it takes to keep them alive.

3. How the Product Get Preserved and Come into Play?

To minimize the decay of activity, we chose the encapsulation techniques——applying a relatively thin coatings to our designed E. coli. When going through harsh gastric environment with stomach acid and bile salts, cells normally lose their activities. However, an embedding layer can let the cells passes through and dissolve after reaching user’s intestine, delivering of our encapsulated freeze-drying strain (Misra et al., 2022).

4. How to Use Our Product?

The nature of this mechanism determines some of the basic instructions, including dosage, storage conditions and shelf life. Dosage: The users will be told to have about 5g of microencapsulated powder and it would be better for the activation if the powder gets dissolved into water or hydromel beforehand. They will be also told not to dissolve the powder into hot water or use antibiotics simultaneously. Moreover, they should take in rhamnose to ensure suicide system works. Storage condition: The consumers will be required to keep our product in a cool and dry place. Our product doesn't need to be freeze. Shelf life: Like other probiotics products, the shelf life will be 24 months under suitable storage conditions.

5. How to Ensure Biosafety?

We have made many improvements to avoid potential risks. As mentioned above, to eliminate indefinite factor, we chose a widely used culture——E. coli Nissle 1917. Plus, we fortunately had a standardized production process of culturing. Moreover, to prevent dysbacteriosis, we chose two types kill switch systems, which can be seen in the description page.

6. References

1. Fenster, K., Freeburg, B., Hollard, C., Wong, C., Rønhave Laursen, R., & Ouwehand, A. (2019, March 17). The Production and Delivery of Probiotics: A Review of a Practical Approach. Microorganisms, 7(3), 83. https://doi.org/10.3390/microorganisms7030083
2. Misra, S., Pandey, P., Dalbhagat, C. G., & Mishra, H. N. (2022, January 30). Emerging Technologies and Coating Materials for Improved Probiotication in Food Products: a Review. Food and Bioprocess Technology, 15(5), 998–1039. https://doi.org/10.1007/s11947-021-02753-5
3. Wu, Y. (2006, August 19). Overweight and obesity in China. British Medical Journal, 333(7564), 362–363. https://www.jstor.org/stable/40699587