Name | Type | Number |
---|---|---|
P2Y2-P2A-Galpha | Coding | BBa_K4220007 |
SST2up-GPD+HygR-SST2down | Composite | BBa_K4220028 |
STE2up-GPD+NeoR-STE2down | Composite | BBa_K4220029 |
1. STE2up-GPD+NeoR-STE2down (“BBa_K4220029”):
a) Function: knock out STE2
b) Purpose: ensure the pheromone α can no longer stimulate the downstream pathway
2. P2Y2-P2A-Galpha (“BBa_K4220007”):
a) Function: express P2Y2 and Galpha (chimeric) at the same time
b) Purpose: replace the ste2 (original GPCR in yeast, activated by pheromone α) into P2Y2 (human GPCR, activated by eATP)
3. SST2up-GPD+HygR-SST2down (“BBa_K4220028”):
a) Function: knock out SST2
b) Purpose: intensify the signal output
1. Original mating pathway:
2. Reprogrammed mating pathway: