Part Collection
Name Type Number
P2Y2-P2A-Galpha Coding BBa_K4220007
SST2up-GPD+HygR-SST2down Composite BBa_K4220028
STE2up-GPD+NeoR-STE2down Composite BBa_K4220029
By using this part collection, we can successfully reprogram mating pathway upstream:

1. STE2up-GPD+NeoR-STE2down (“BBa_K4220029”):

a) Function: knock out STE2

b) Purpose: ensure the pheromone α can no longer stimulate the downstream pathway

2. P2Y2-P2A-Galpha (“BBa_K4220007”):

a) Function: express P2Y2 and Galpha (chimeric) at the same time

b) Purpose: replace the ste2 (original GPCR in yeast, activated by pheromone α) into P2Y2 (human GPCR, activated by eATP)

3. SST2up-GPD+HygR-SST2down (“BBa_K4220028”):

a) Function: knock out SST2

b) Purpose: intensify the signal output

Illustration:

1. Original mating pathway:

2. Reprogrammed mating pathway: