We have improved a kill switch in E. coli (composite part) into one which can function in the yeast by replacing each basic parts into ones which have similar functions in the yeast.
a) Improvement of basic parts:
| Type | Number |
|---|---|
| Promoter | HIS3 |
| Coding | NatR |
| Terminator | ADH1 |
*PyeaR is a promoter in E. coli which is responsive to nitrate, nitrite and nitric oxide.
*CUP1 is a promoter in yeast which is responsive to copper ion.
b) Adding resistant genes:
| Function | Original (E. coli) | New (Yeast) |
|---|---|---|
| Promoter | PyeaR | CUP1 |
| Essential gene | SerA | HSF1 |
c) Rearrangement and design:
