Overview
BioBricks have always been the foundation for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. After getting the parts we want, we used PCR amplification with our constructed expression plasmids as templates to construct multiple BioBricks. For the basic parts, each part contains a coding sequence, while the composite parts are extended with promoters, RBS, and terminator. During our MerSe project, NCKU_Tainan created a part collection comprised of 11 basic parts and 11 improved composite parts. All the basic parts and composite parts are included in our Part Collection as the comprehensive system for Sec and selenomelanin biosynthesis.
Basic Parts
Name | Description | Type | Length |
BBa_K4171000 | CysK | Coding | 972 bp |
BBa_K4171001 | SerRS | Coding | 1293 bp |
BBa_K4171002 | SelA | Coding | 1392 bp |
BBa_K4171003 | SelD | Coding | 1044 bp |
BBa_K4171004 | CysE* | Coding | 828 bp |
BBa_K4171005 | YdeD | Coding | 900 bp |
BBa_K4171007 | tRNA(Ser) | RNA | 88 bp |
BBa_K4171008 | tRNA(Sec) | RNA | 95 bp |
BBa_K4171009 | tRNA(UTuX) | RNA | 91 bp |
BBa_K4171025 | Promoter for tyrP | Regulatory | 29 bp |
BBa_K4171026 | RBS for tyrP | RBS | 15 bp |
Composite Parts
Name | Description | Type | Length |
BBa_K4171010 | PlacI-cysK | Composite | 1198 bp |
BBa_K4171011 | PlacI-cysE* | Composite | 1056 bp |
BBa_K4171012 | PlacI-cysK-cysE* | Composite | 2034 bp |
BBa_K4171013 | PompA-ydeD | Composite | 1229 bp |
BBa_K4171014 | PT7-tRNA(Ser) | Composite | 119 bp |
BBa_K4171015 | PT7-tRNA(Sec) | Composite | 126 bp |
BBa_K4171016 | PT7-tRNA(UTuX) | Composite | 122 bp |
BBa_K4171018 | Ptrc-melA | Composite | 1933 bp |
BBa_K4171019 | Ptrc-melA-B0015 | Composite | 2070 bp |
BBa_K4171023 | Ptrc-gadB-B0015 | Composite | 1622 bp |
BBa_K4171024 | NPS-tyrP-Ptrc-melA-B0015 | Composite | 3435 bp |