2022/5/30

2022/6/6

2022/6/13

2022/6/20

2022/6/27

2022/7/4

2022/7/11

2022/7/18

2022/7/25

2022/8/1

2022/8/8

2022/8/15

2022/8/22

2022/8/29

2022/9/5

2022/9/12

2022/9/19

Wet

  1. Construct plasmid
  2. Design primers for tRNA
  3. Construct the sequences of tRNA
  1. Construct the pSU-cysK plasmid
  2. Design primers for tRNASer, tRNASec, and tRNAUTuXconstruction
  3. Construct the sequences of tRNASer and tRNASec with T7 promoter into TA vector

Dry

  1. Hardware brainstorming
  2. Meet with experts
  3. Study the basic knowledge of modeling
  1. Hardware brainstorming
  2. Meet with experts to ask for advices on the hardware ideas
  3. Study the enzyme kinetics and other basic knowledge of modeling

HP

  1. Smart community workshop
  2. ARS preparation
  3. Fortnightly and talk show preparation
  1. Participate in Smart community workshop
  2. ARS preparation
  3. Prepare for the second episode of the fortnightly and the talk show
  4. Discuss collaboration details with NCHU

Wet

  1. Construct plasmid
  2. Design primers for tRNA
  3. Construct the sequences
  1. Construct the pSU-cysK plasmid
  2. Design primers for tRNASer, tRNASec, and tRNAUTuX
  3. Construct the sequences of tRNASer and tRNASec with T7 promoter into TA vector

Dry

  1. Hardware brainstorming
  2. Meet with experts
  3. Search for information
  1. Hardware brainstorming
  2. Meet with experts to ask for advices on the hardware ideas
  3. Search for useful model information regarding our project

HP

  1. ARS preparation
  2. Fortnightly and talk show preparation
  3. Post team member introduction
  1. ARS preparation
  2. Prepare for the second episode of the fortnightly and the talk show
  3. Post team member introduction on Instagram
  4. Discuss collaboration details with NCHU

Wet

  1. Construct plasmids
  2. Perform PCR to copy tRNA sequences and condense the PCR results
  1. Construct pSU-cysK-cysE* and pSU-cysE* plasmids
  2. Perform PCR to copy tRNASer, tRNASec, tRNAUTuX DNA sequences on TA vector and condense the PCR results

Dry

  1. Meet with experts
  2. Research
  3. Browse previous iGEM team Best Model
  1. Meet with experts
  2. Research on all kinds of microgravity simulators
  3. Browse previous iGEM team Best Model winner teams' wiki
  4. Learn wiki editting

HP

  1. Logo design
  2. Entrepreneurship design
  3. ARS and talk show preparation
  1. Logo design
  2. Entrepreneurship design
  3. ARS and talk show preparation
  4. Education kit design

Wet

  1. Sequencing of cysE* and ydeD
  2. Check cysE* function
  3. Synthesize RNA
  1. Do sequencing to check the structures of cysE* and ydeD
  2. Check cysE* function by transforming pSU-cysE* into △cysE and measure the OD value
  3. Synthesize RNA in vitro

Dry

  1. Meet with experts
  2. Start building hardware
  3. Design Wiki page
  1. Meet with experts
  2. Start building UV stage and designing clinostat
  3. Determine the main colors of Wiki page

HP

  1. Promotion video preparation
  2. Logo design
  3. ARS and talk show preparation
  1. Promotion video preparation
  2. Logo design
  3. ARS and talk show preparation
  4. Education kit, literature, entrepreneurship, activity design

Wet

  1. Purify SerRS, SelA and SelD
  2. Synthesize RNA
  1. Purify SerRS, SelA and SelD with His-tag
  2. Synthesize RNA

Dry

  1. Software idea discussion
  2. Building hardware
  3. Reading papers
  1. Software idea discussion
  2. Build the first prototype of UV stage
  3. Reading papers regarding the melanin pathway
  4. Determine the Wiki page structure and design the layout

HP

  1. Logo design
  2. Collaboration discussion
  3. Literature, activity design
  1. Logo design
  2. Collaboration discussion
  3. Literature, activity design
  4. Talk show preparation

Wet

  1. Transform ydeD
  2. Measure the melanin production
  3. Construct backbones
  1. Transform ydeD into MG1655 and △iscS
  2. Measure the melanin production under different temperatures and mediums
  3. Construction of different backbone of plasmids

Dry

  1. Build clinostat
  2. Consult Landing AI
  3. Read papers for modeling
  1. Build clinostat
  2. Consult Landing AI about software design
  3. Read papers regarding the selenocysteine pathways
  4. Design Wiki layout

HP

  1. Audiobook design
  2. Discuss collaboration
  3. Visit National Academy of Taiwan
  1. Audiobook design
  2. Discuss the collaboration details with other schools
  3. Visit National Academy of Taiwan
  4. Discuss entrepreneurship details with Gran Systems

Wet

  1. Transform pcysK-cysE*
  2. Reanneal the synthesized RNA
  3. Run SDS-PAGE
  1. Transform pcysK-cysE* into MG1655 and △iscS with and without ydeD
  2. Reanneal the synthesized RNA
  3. Run SDS-PAGE to analyze temperature effects on protein expression of melA
  4. Test the survival rate of the bacteria with melanin under UV radiation

Dry

  1. Build clinostat
  2. Software design
  3. Read papers
  1. Build clinostat and encounter some difficulties
  2. Software design
  3. Read papers regarding the selenocysteine pathway
  4. Start editing the Wiki templates

HP

  1. Audiobook design
  2. Find sponsorship
  3. Entrepreneurship, education kit design
  1. Audiobook design
  2. Find sponsorship
  3. Entrepreneurship, education kit design
  4. Talk show preparation

Wet

  1. Compare the growth rate
  2. Condense SerRS, SelA and SelD
  1. Compare the growth rate of MG1655 and △iscS with constructed plasmids
  2. Condense SerRS, SelA and SelD; run SDS-PAGE with Western blotting and coomassie blue staining

Dry

  1. Discuss about microfluidic chip
  2. Start constructing the software
  3. Modeling the melanin pathway
  1. Discussion about the idea of microfluidic chip
  2. Start constructing the software
  3. Modeling the melanin pathway
  4. Wiki page editing and art design

HP

  1. Promotion video preparation
  2. Discuss the collaboration details with other schools
  3. Literature, slides and activity design
  1. Promotion video preparation
  2. Discuss the collaboration details with other schools
  3. Literature, slides and activity design
  4. Talk show 2.0 preparation

Wet

  1. Aminoacylation of tRNA and serine
  2. Conversion of seryl-tRNA
  3. Compare the function
  1. Aminoacylation of tRNA and serine
  2. Conversion of seryl-tRNA to selenocysteinyl-tRNA
  3. Compare the function among pSB4KI-Ptrc-melA and pSB4KI-Ptrc-melA-B001

Dry

  1. Learn basic knowledge and visit NHRI
  2. Present our modeling idea to professors
  3. Use AI platform for software model training
  1. Learn the basic knowledge of microfluidic chip and visit NHRI to fabricate our first version of microfluidic chips
  2. Present our modeling idea to professors and discover some problems to be solved
  3. Use AI platform for software model training
  4. Wiki page editing and art design

HP

  1. Audiobook recording
  2. Promotion video preparation
  3. Wiki page, IHP content discussion
  1. Audiobook recording
  2. Promotion video preparation
  3. Wiki page, IHP content discussion
  4. Talk show 2.0 preparation

Wet

  1. Test different sodium selenite concentrations
  2. Deacylate selenocysteine
  3. Transform gadB
  1. Culture pcysK-cysE*, pPompA-ydeD/MG1655 in different sodium selenite concentrations.
  2. Deacylate selenocysteine from tRNA
  3. Transform gadB to E. coli to produce GABA
  4. Compare the function of melanin production between DH5a, MG1655, BL21, and W3310

Dry

  1. Design the second prototype of UV stage
  2. Software model training
  3. Learn Copasi and MATLAB
  1. Design the second prototype of UV stage
  2. Continue with software model training
  3. Learn how to use Copasi and MATLAB for pathway simulation
  4. Build our melanin pathway model
  5. Discuss hanging drop simulation
  6. Wiki page editing and art design

HP

  1. Audiobook recording
  2. Promotion video preparation
  3. Wiki page, IHP content discussion
  1. Audiobook recording
  2. Promotion video preparation
  3. Wiki page, IHP content discussion
  4. Literature design
  5. Talk show 2.0 preparation

Wet

  1. Compare melanin production
  2. Test different substrates for GABA production
  3. Conversion of seryl-tRNA
  1. Compare melanin production with and without TyrP between DH5a, MG1655, and BL21
  2. Measure the production of GABA under different substrates.
  3. Conversion of seryl-tRNA to selenocysteyl-tRNA

Dry

  1. Microfluidic chip fabrication
  2. Software coding
  3. Selenocysteine pathway model
  1. Visit NHRI to fabricate the microfluidic chip with different well diameter for future testing and improvement
  2. Start coding for our software
  3. Build our two selenocysteine pathway model
  4. Finish the art design of the Wiki main page, Team page

HP

  1. Audiobook recording
  2. Promotion video preparation
  3. Wiki page, IHP content discussion
  1. Audiobook recording
  2. Promotion video preparation
  3. Wiki page, IHP content discussion
  4. Literature design
  5. SDG poster design
  6. Talk show 2.0 preparation

Wet

  1. UV experiments
  2. Construction terminator and promoter
  3. Remove KmR
  1. Test the melanin function by UV experiments
  2. Constuction of rrnC terminator and lpp promoter
  3. Remove KmR from ΔtyrA

Dry

  1. Survival rate experiment
  2. Taguchi experiments
  3. Medium exchange model
  1. Survival rate experiment in MerStage
  2. Finish Taguchi experiments
  3. Learn how to use Ansys Fluent to simulate medium exchange in the microfluidic chip
  4. Wiki animation coding

HP

  1. Talk show 2.0
  2. Discuss with companies
  3. Collaboration with other schools
  1. Talk show 2.0
  2. Discuss with companies
  3. Collaboration with other schools

Wet

  1. Dual plasmids GABA production
  2. Deacylation
  3. Amino acid purification
  1. Measure the production of GABA from dual plasmids
  2. Deacylation with nuclease S1 and Tris-HCl
  3. Amino acid purification
  4. Confirm the production of selenocysteine by HPLC

Dry

  1. Survival rate experiment
  2. Confirmation experiments
  3. Cell aggregation model discussion with experts
  1. Survival rate experiment in MerStage
  2. Finish confirmation experiments for Taguchi methods
  3. Discussion with Prof. Wei about nutrient consumption rate calculation and cell aggregation model
  4. Wiki animation coding

HP

  1. Promotion video
  2. Discuss with companies
  3. Collaboration with other schools
  1. Finich making promotion video
  2. Discuss with companies
  3. Collaboration with other schools

Wet

  1. Protocol adjustment
  2. Urea PAGE
  3. Confirm YdeD function
  1. Adjustment of digestion and ligation protocol
  2. Apply Urea PAGE
  3. Use CFU to confirm YdeD function
  4. Confirm the production of selenocysteine by HPLC

Dry

  1. Discuss different fabrication methods
  2. Survival rate experiment
  3. Cell aggregation model
  1. Discussion with Prof. Juang about different chip fabrication methods
  2. Survival rate experiment in MerStage
  3. Finish construction of cell aggregation model
  4. Wiki debug

HP

  1. Workshop preparation
  2. Wiki page writing
  3. Wiki page adjustments
  1. Workshop preparation
  2. Wiki page writing
  3. Wiki page adjustments
  4. Literature design

Wet

  1. Produce selenomelanin
  2. Urea PAGE
  1. Start to produce selenomelanin and compare it with melanin
  2. Urea PAGE method adjustment

Dry

  1. Survival rate experiment
  2. MerStage optimization
  3. Melanin pathway model
  1. Survival rate experiment in MerStage
  2. MerStage optimization
  3. Finish melanin pathway experiments and model
  4. Wiki page content writing and coding

HP

  1. Workshop preparation
  2. Wiki page writing
  3. Wiki page adjustments
  1. Workshop preparation
  2. Wiki page writing
  3. Wiki page adjustments

Wet

  1. Test selenomelanin function
  2. Wiki page writing
  1. Test the function of selenomelanin
  2. Wiki page writing

Dry

  1. Wiki page writing
  1. Wiki page writing

HP

  1. Workshop
  2. Wiki page writing
  3. Wiki page adjustments
  1. Workshop
  2. Wiki page writing
  3. Wiki page adjustments

Wet

  1. Wiki page writing
  2. Wiki page adjustments
  1. Wiki page writing
  2. Wiki page adjustments

Dry

  1. Wiki page writing
  2. Wiki page adjustments
  3. Wiki debug
  1. Wiki page writing
  2. Wiki page adjustments
  3. Wiki debug

HP

  1. Wiki page writing
  2. Wiki page adjustments
  1. Wiki page writing
  2. Wiki page adjustments

ALL

WET

DRY

HP

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