Bronze

I. Registration and Giant Jamboree Attendance.

    All members of the team have registered as an undergraduate team in iGEM 2022.

II. Competition Deliverables

    According to the Deliverables page on iGEM’s website:

1. We have created a wiki page, which includes an Attribution page. | NCKU_Tainan - iGEM 2022 - Attribution
2. We have produced a promotion video for our project this year, see it on our Main Page
3. We have prepared for the team presentation during the Jamboree.
4. We have submitted the Judging form and safety forms before the deadline. | Team:NCKU_Tainan/Judging Form (igem.org)

III. Project Attributions

    We have an Attribution page for whom that has helped us along our iGEM journey. | NCKU_Tainan - iGEM 2022 - Attribution

IV. Project Description

    Our project aimed to develop a biomanufacturing tool that can resist strong radiation and produce precious substances in space. To get further information about the background and inspiration, please look at our Description page. | NCKU_Tainan - iGEM 2022 - Description

V. Contribution

    NCKU_Tainan has made five achievements to contribute to future iGEM teams, including innovative Sec synthesis pathway, optimization of melanin production by Taguchi method, the hardware, MerStage, etc. To get further information about the background and inspiration, please look at our Contribution page. | NCKU_Tainan - iGEM 2022 - Contribution

Silver

I. Engineering Success

    NCKU_Tainan has successfully synthesized the 21^st amino acid, selenocysteine (Sec), through the iterative Design-Build-Test-Learn cycles. For more details, please look at our Engineering page. | NCKU_Tainan - iGEM 2022 - Engineering

II. Collaboration

    iGEM allowed us to meet and engage with students that have similar motivations. For example, coloring books and podcasts were produced with Tec-Chihuahua and KU_Leuven for a wider audience from different backgrounds. NCKU_Tainan has built meaningful bonds with other iGEM teams and shaped both teams' projects in the journey. For more details, please look at the Collaboration page. | NCKU_Tainan - iGEM 2022 - Collaboration

III. Human Practices

    The three main focuses of HP are assuring the value of the project, realizing how it can be responsible, and reaching out to any communities directly or indirectly influenced by this issue. To achieve this, NCKU_Tainan followed the framework: Anticipation, Reflection, Deliberation, and Responsiveness. For more details, please look at the Human practice page. | NCKU_Tainan - iGEM 2022 - Human Practices

IV. Proposed Implementation

    Our ultimate goal was to bring our project into real life. Therefore, in the proposed implementation, we described how we would apply each aspect of our project in the real world. For more details, please look at the Implementation page. | NCKU_Tainan - iGEM 2022 - Implementation

Gold

I. Integrated Human Practices

    In response to the design of the Silver medal Human Practices, NCKU_Tainan communicated with experts, stakeholders, and people from various fields to determine the project's value, responsibility, risks and impacts. All the reflection during the process is integrated, thus turning the idea into a potential product. For more details, please look at the Human Practices page. | NCKU_Tainan - iGEM 2022 - Human Practices

II. Improvement of an Existing Part

    We improved the biobrick BBa_K274001 from 2009 iGEM Cambridge team by adding tyrP gene, which encodes for tyrosine transporter protein. With the addition of TyrP, E. coli was allowed to have higher tyrosine uptake. For more information, please look at the Improvement page. | NCKU_Tainan - iGEM 2022 - Improvement

III. Project Modeling

    NCKU_Tainan aims to maximize melanin production to protect bacteria from radiation and introduces four models into the project, including Taguchi methods and Melanin Production Model to optimize melanin yield, and predict melanin production, Nutrient Uptake Model and Trasport Model for Bacterial Growth to describe bacteria accumulation in a quantitative way. For more details, please look at the Model page. | NCKU_Tainan - iGEM 2022 - Model

IV. Proof of Concept

    Se coli is an engineered E. coli synthesizing selenomelanin, an ultra-robust biochemical against radiation. Selenomelanin is from selenocysteine, the 21^st amino acid, and MelA for melanin. NCKU_Tainan has designed in vitro and in vivo pathways for Sec production as proof of concept. For more details, please look at the Proof of Concept page. | NCKU_Tainan - iGEM 2022 - Proof of Concept

V. Partnership

    With CCU_Taiwan throughout the year, we have established a firm partnership and helped each other to refine the projects. We met each other through an online meeting and the ARS (AFOB Regional Symposium) conference. Further collaborations were followed by the the TCUS cross-disciplinary career competition held by CCU_Taiwan. For more details, please look at the Partnership page. | NCKU_Tainan - iGEM 2022 - Partnership

VI. Education & Communication

    NCKU_Tainan has been dedicated to designing comprehensive and innovative activities to engage people with different identities in the science community, including audiobooks, a workshop and three original talk shows. For more details, please look at the Communication page. | NCKU_Tainan - iGEM 2022 - Education & Communication