Introduction
As you know, in synthetic biology we use a transcriptional unit in order to change the gene expressions in living beings. So this year we have uploaded 11 different constructions to the Part Pages Registry.
| Name | Promoter | Type | Origin | Location |
|---|---|---|---|---|
| BBa_K4374000 | ACO3 | Plasmid | A. thaliana | TCA cycle |
| BBa_K4344001 | ADH1 | ADH1 | A. thaliana | Glycolysis |
| BBa_K4374002 | ALDH2 | Plasmid | A. thaliana | Glycolysis |
| BBa_K4374003 | ATPS | Plasmid | A. thaliana | Respiratory chain |
| BBa_K4374005 | CI76 | Plasmid | A. thaliana | Respiratory chain |
| BBa_K4374006 | CIII 14 | Plasmid | A. thaliana | Respiratory chain |
| BBa_K4374007 | CSY4 | Plasmid | A. thaliana | TAC cycle | BBa_K4374008 | G3PDH1 | Plasmid | A. thaliana | Glycolysis |
| BBa_K4374009 | HB1 | Plasmid | A. thaliana | - |
| BBa_K4374010 | HB2 | Plasmid | A. thaliana | - |
| BBa_K4374011 | PEPC1 | Plasmid | A. thaliana | Glycolysis |
Main construction
Using the Gateway Method, we have developed 11 plasmids which are very similar. All of them present a promoter, a gene of interest, a terminator and a gene of resistance to an antibiotic. But if they all seem to be the same, what are the differences?
The answer is the promoter. Each one of the eleven plasmids has got a different promoter, like this we are able to do the experiment as standardized as possible because we only wanted to see the promoter's gene induction.
The gene of interest is the GFP, the terminator is the TNos and the antibiotic resistance is Kanamycin.
| Promoter | Plasmid |
|---|---|
| BBa_K4374000 |  |
| BBa_K4374001 |  |
| BBa_K4374002 |  |
| BBa_K4374003 |  |
| BBa_K4374005 |  |
| BBa_K4374006 |  |
| BBa_K4374007 |  |
| BBa_K4374008 |  |
| BBa_K4374009 |  |
| BBa_K4374010 |  |
| BBa_K4374011 |  |