Model

Modeling


1. Simple Model


matlab code

Result


Since the same CFE expression vector and the same CFE E.coli expression system are used, the expression patterns for the two parts will be the same.
The difference in the final yield of enzymes will depend on the expression rate or degradation rate according to the sequence length of each gene.

2. Complex Model


It can be seen that the rate constant is temperature dependent.
In the case of pT7, it can be confirmed that protein production is significantly higher at 25 degrees than at 4 degrees.
And theoretically, it can be seen through the corresponding equation.


References


  • http://parts.igem.org/Help:Cell-free_chassis/Modelling#References
  • https://www.jbc.org/article/S0021-9258(20)68115-7/fulltext