Overview
Our "intelligent box" (i-Box) includes a blood collection module and a triplamide oxide (TMAO) detection module. We use a color recognition detection method to quantify the concentration of trimethylamine oxide and give a therapeutic diagnosis and a recommended dose of our product. We connect the smartphone to the "box". Users can know the condition in real time by drawing blood, determine the rationality of the current treatment plan, and make our hardware closer to life.
Design
"i-Box"
In our "i-Box", we designed two modules: blood acquisition module and TMAO detection module.
1.Blood detectionmodule
Users use 28G blood glucose needles(Figure 1.) to extract trace amounts of blood. In order to isolate plasma and blood cells and prevent the impact of blood cells on TMAO detection in the blood, we added a separation pad to the blood glucose needle to filter blood cells in the blood. When the blood glucose needle is flipped, the liquid to be tested will flow into the blood glucose needle collection end.
2.TMAO detection module
In this module, we have set up four cells (cavity) (Figure 2.) to separate different operations. We call them as reaction&waste liquid cell,supplementary liquid cell,color recognition cell,Bluetooth transfer cell.
Reaction&waste liquid cell is divided into upper and lower layers, and the upper stores reaction liquid, the waste liquid flows out from below. The reaction liquid consists of 100μLH20, 20μLHCl, 20μLpolylamine hydrochloride-capped manganese dioxide nanoparticles (PAH@MnO2NPs) , the supplementary liquid cell connected with the reaction&waste liquid cell by a speed limit valve. According to the relevant literature [1], the interaction between TMAO and the reaction liquid will produce a color reaction. When the content of TMAO increases from 0, the reaction liquid will change from yellow to light blue. Therefore, the color recognition cell is used to identify color changes through photos, which are transmitted to the APP terminal through Bluetooth transfer cell.
"APP"
We have developed a multi-functional APP to connect the box and analyze the data. The APP is used to control the speed limit valve (access and discharge of the reaction liquid), identify the color of the TMAO reaction liquid, and provide follow-up treatment solutions...
For more details, please click the following link https://2022.igem.wiki/hzau-china/software.
In a word, we injected the blood sample into the reaction solution. After a period of reaction, the photos were uploaded to the mobile phone, and the APP was used to analyze the color value to determine the TMAO content in human blood and the follow-up treatment plan.