This year, all iGEMers from HZAU-China have actively participated in the various templates of this project, and each member has demonstrated their unique talents in the preparation of this competition. In addition, we received a lot of help, from brainstorming to closing the project completely. All the work done and the help received are shown below.
Project
The idea of this project is derived from the story of Xue Zhang in her life. Xue Zhang, Jie Min, Mingqin Yang and Ruixuan Zhu provided clear direction for the design of the project, and other members provided a lot of literature as support for the project and provided suggestions.
Team Training
Experimental education for new team members was conducted by Xue Zhan, Jie Min, Huiling Lu. Modeling training for new team members was conducted by Yini Xiao, Ziyue Li, Yu Zhang. Safety education was conducted by Professor Chunjiao Xia from Huazhong Agricultural University. Our advisor Yifei Wang is a postgraduate who gave us a lot of practical techniques in complicated molecular cloning and experimental design. Our instructor Professor Jin He, Binguang Ma, Xun Wang, Wei Xu provided countless practical advices in our weekly group meeting to help us solve experimental difficulties.
Resources
tmd, dmd, loxp, nattokinase were synthesized by GenScript.
Theophylline-induced riboswitch was obtained from our Professor Xun Wang. The plasmid carrying the Cre sequence was from our special instructor: Yifei Wang.
A loxp component comes from our partner , Zhejiang University of Technology.
The green fluorescent protein reporter gene was from HZAU-China 2021.
The red fluorescent protein reporter gene was obtained from the iGEM plamisd kit.
SnapGene® licenses were generously provided by GSL Biotech LLC.
The appratatus of GC and HPLC were supported by experimental appratatus administrator Jinshan Li.
Wiki
Codes on our wiki were written by Junmin Han, Zhilin Zhu, Xianxin Huang, Mingyu He.
Art design on the wiki was fulfilled by Anqi Chen.
Hardware & software
Hardware box was designed and manufactured by Yini Xiao, Junmin Han and Zhaohong Liu.
Software "APP" was programed by Xianxin Huang，Junmin Han.
Experiments
Jie Min, Yutong Chen, and Rui Tian were in charge of the degradation module, Ruixuan Zhu was in charge of the cre-loxp module, Meng Sun and Sijia Xu were in charge of the suicide module, Xue Zhang and Huiling Lu were in charge of the alleviation module.
Human Practices
Xiaojin Chen took charge in HP Group and supervised all HP activities. The Wechat tweets, interviews were made by all HP members together. The planning of human practice activities were conducted by all members. Intestinal information platform was conducted by Xiaojin Chen. Conghao Qin present the opinion to the government. Probiotics Handbook was mainly made by Bijun Ling, Xiaojin Chen, Huayi Feng. Additional assistance from Yuqi Ren.
Model
Yini Xiao took charge in Model Group. The degradation model was finished by Yini Xiao ,Yan Xu and Yu Zhang. The point mutation model was finished by Ziyue Li ,Zhaohong Liu, and Leyang Huang, assisted by Yini Xiao ,Yu Zhang and others. The Virtual screening model was finished by Yini Xiao and Yu Zhang.
Daily Management
Huiling Lu, Jie Min and Mingqin Yang took responsibility of purchasing experimental materials and reagents. Huiling Lu, Jie Min took responsibility of the financial affairs on experiment equipments and reagents. Daily cleaning of our laboratory was assigned to all team members.